培养基中添加植物生长调节剂的蛹虫草转录组学分析 (1).pdfVIP

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培养基中添加植物生长调节剂的蛹虫草转录组学分析

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王哲，王升厚，李梦雪，柳叶飞，王泽，徐方旭*

1.沈阳师范大学生命科学学院（沈阳110034）；2.沈阳师范大学实验教学中心（沈阳110034）

摘要为探究培养基中添加植物生长调节剂(GN-01)对蛹虫草基因表达变化的影响,采用高通量测序技术对培

养基中添加GN-01栽培的蛹虫草和对照组蛹虫草鲜品进行转录组测序分析,采用生物信息学方法对差异基因进行

功能注释和通路分析。结果表明:培养基中添加GN-01后,蛹虫草子实体中显著差异基因有189个,共富集到29条代

谢通路;差异基因主要参与次生代谢产物的生物合成、精氨酸和脯氨酸的代谢、植物过氧化物酶体路径。试验证

实GN-01能够激活与抗病能力有关代谢通路,在提高蛹虫草抗病效应中发挥重要作用。

关键词蛹虫草;寄生性病原真菌;植物生长调节剂;转录组学;差异表达基因

TranscriptomicsAnalysisofCordycepsmilitariswithPlantGrowth

RegulatorAddedtoCultureMedium

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WANGZhe,WANGShenghou,LIMengxue,LIUYefei,WANGZe,XUFangxu*

1.CollegeofLifeScience,ShenyangNormalUniversity(Shenyang110034);

2.ExperimentalTeachingCenter,ShenyangNormalUniversity(Shenyang110034)

AbstractToexplorethemediumaddingplantgrowthregulator(GN-01)ongeneexpressionchangesandtheeffectof

Cordycepsmilitarisusinghigh-throughputsequencingtechnologytoadddesigned,theGN-01cultivatedCordycepsmilitaris

culturemediumandthecontrolgroupCordycepsmilitarissoldthetranscriptomesequencinganalysis,bioinformaticsmethods

fordifferencesingenefunctionannotationandpathanalysis.Theresultsshowedthat189genesweresignificantlydifferentin

Cordycepsmilitaris,whichwereenrichedinto29metabolicpathways.Differentialgenesweremainlyinvolvedinbiosynthesis

ofsecondarymetabolites,metabolismofarginineandproline,andperoxisomepathwayinplants.Theseresultsindicatedthat

GN-01couldactivatemetabolicpathwaysrelatedtodiseaseresistance