鲍鱼内脏论文：鲍鱼内脏多糖的结构和活性研究.pdfVIP

鲍鱼内脏论文：鲍鱼内脏多糖的结构和活性研究

【中文摘要】从鲍鱼内脏中提取活性多糖,为提高鲍鱼内脏的附

加值,促进鲍鱼的综合利用和开发提供理论和技术支持。本论文采用

水煮、醇沉的方法,得到鲍鱼内脏粗多糖CAVP,CAVP经冻融分级、酶

-Sevag法联合脱蛋白、SepharoseCL-6B柱层析等方法进行纯化,得到

水溶性多糖AVP,苯酚-硫酸法测总糖含量为91.24%。经

SepharoseCL-6B柱层析和HPLC分析,AVP为均一性多糖,分子量约为

3.5万。气相色谱法分析表明AVP主要由Man、Gal、Glc三种单糖组

成,其摩尔比为2.7:0.7:6.5。采用部分酸水解、高碘酸氧化、Smith

降解、甲基化及IR、GC、GC-MS等方法对AVP的结构进行分析。AVP

经部分酸水解反应,AVP的主链由Glc单糖组成,糖链末端或支链主要

由Glc和Gal组成,支链或主链边缘主要是Man。AVP甲基化分析结

果表明,AVP主链部分主要由(1→4)-Glc组成,分支点为Glc的6-O处,

分支点残基为(1→4,6)-Glc;支链部分主要是(1→3,6)-Man;非

【英文摘要】Weextractedactivepolysaccharidefrom

Abaloneviscerawhichcanprovidetheoreticalandtechnical

supporttoincreasetheaddedvalueofabaloneviscera,andto

promotetheutilizationanddevelopmentofabalone.The

polysaccharideofCAVPwasisolatedfromAbalonevisceraby

water.Thewater-extractedpolysaccharideCAVPwas

fractionatedbyfrozen-thawed,deproteinatedwith

enzyme/Sevagmethodandpurifiedbygelfiltration

chromatographyonacolumnofSepharoseCL-6B.Thenfraction

wereobtainedandnamedasAVP.Thecontentofpolysaccharide

was91.24%.Themolecularweightofitwas3.5×104Da,which

measuredbyHPLC.GCanalysisshowedthatAVPwascomposedof

Man,GalandGlc.Themolarratiowas2.7:0.7:6.5.Thestructure

ofAVPwaselucidatedbyperiodateoxidation,Smithdegradation,

methylationanalysisandIR,GC,GC-MS.Thepartialacid

hydrolysisofAVPshowedthatthemainchainofAVPwascomposed

ofGlc.Theendofsugarchainorbranched-chainwascomposed

ofGalandGlc.ThemethylationanalysisshowthatAVPconsisted

ofabackbonewascomposedof(1→4)-GlcThesidechainswas

composedof(1→3,6)-linked–Mannon-reducingterminal

residueswascomposedof(1→)-Galand(1→)-Glc.The

immunobiologicalactivityofCAVPandAVPweredetectedby

Lymphoc