基于免疫基因组学建立肝内胆管癌患者的预后风险模型.pdfVIP

基于免疫基因组学建立肝内胆管癌患者

的预后风险模型

摘要:目的:利用生物信息学方法，筛选影响肝内胆管癌预后的免疫基因，构

建肝内胆管癌患者的免疫预后模型。方法:利用TCGA中肝内胆管癌患者的RNA表

达谱及相应的临床资料，和ImmPort数据库中的免疫基因数据。建立免疫预后风

险评估模型。采用Kaplan-Meier生存分析和受试者工作特征(ROC)曲线对免疫基

因预后模型的预测能力进行评价。结果:肝内胆管癌和癌旁组织中共筛选出418

个差异表达的免疫基因。Cox回归分析表明DMBT1,IGF1,SLIT2,PLAU,ESM1,

CDH1，VIM,A2M,ULBP2,UCN2,CCL13,IL11等12个免疫基因与预后相关。肝

内胆管癌中高风险组的预后明显低于低风险组（P0.001），ROC曲线下面积为

0.943。结论:构建了肝内胆管癌的免疫基因预后模型，该模型可能成为一种有前

景的预测生物标志物，为肝内胆管癌的个体化治疗提供新的视野。

关键词:肝内胆管癌；免疫基因；预后模型

Developmentofaprognosticriskmodelforpatientswith

intrahepaticcholangiocarcinomabasedonimmunogenomics

CuiMeng,YangJia,ShuiXin,LiaoDan,Chenjunliang

(DepartmentofClinicalLaboratory,LeshanPeoplesHospital,

Leshan,Sichuan614000,China)

Objective:Theimmunoprognosticmodelofintrahepatic

cholangiocarcinomawasestablishedbyscreeningtheimmunegenesthat

affecttheprognosisofintrahepaticcholangiocarcinomaby

bioinformaticsmethod.Methods:RNAexpressionprofileandclinical

dataofpatientswithintrahepaticcholangiocarcinomainTCGAand

immunegenedatainImmPortdatabasewereused.Toestablishanimmune

prognosticriskassessmentmodel.Kaplan-meiersurvivalanalysisand

receiveroperatingcharacteristic(ROC)curvewereusedtoevaluate

thepredictivepoweroftheimmunegeneprognosticmodel.Results:A

totalof418differentiallyexpressedimmunegeneswerescreenedout

fromintrahepaticcholangiocarcinomaandparacancertissues.Cox

regressionanalysisshowedthatDMBT1,IGF1,SLIT2,PLAU,ESM1,CDH1,

VIM,A2M,ULBP2,UCN2,CCL13,IL11werecorrelatedwithprognosis.The

prognosisofintrahepaticcholangiocarcinomainthehigh-riskgroup

wassignificantlylowerthanthatinthelow-riskgroup(P0.001),and

theareaunderROCcurvewas0.943.Conclusion:Theimmunogenetic

prognosticmodelofintrahepaticcholangiocarcinomaiscons