稀有鮈鲫_一种新的鱼类实验动物 .pdfVIP

实验动物科学与管理

·96·LABORATORYANIMALSCIENCEANDMANAGEMENT2003年第20卷增刊

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AnotherNovelTestfortheDetectionofPyrogeninOrdertoReplacetheRabbit

PyrogenTestbyDeterminationoftheReleaseofTNF-αfromTHP-1Lines

HUANGQing-quan,LIGuan-min,XITing-fei,HEZheng-ming

(NationalInstitutefortheControlofPharmaceuticalandBiologicalProducts,Beijing100050,China)

Abstract:Theaimofthisstudywastodevelopmentaninvitrotestforthedetectionofpyrogeninordertoreplacethe

rabbitpyrogentest.RecentlytheTHP-1monocyticcellswasassessedfortumornecrossfactor(TNF)-αfortheprobability

ofthenoveltest.WhenTHP-1linesisincubatedinthepresenceofreferencestandardendotoxin,theysecret