三甲氧基二苯乙烯对小鼠巨噬细胞产肿瘤坏死因子-α及细胞核因子-κB 活.pdfVIP

三甲氧基二苯乙烯对小鼠巨噬细胞产肿瘤坏死因子-α及细胞

核因子-κB活性的作用研究

曾清华;饶慧;高洁生

【摘要】ObjectiveTostudytheregulatingeffectsof3,5,4-

trimethoxystilbene(BTM)ontheTNF-αproductionandtheNF-κB

activationinmurinemacrophagesactivatedbyLPS.MethodsThemurine

macrophagecelllineRAW264.7cellswereculturedanddif-ferent

concentrationsofBTMorresveratrolwereadded,thenLPSactivatedcells

wereadded.ThecellularsupernatantwascollectedfordetectingtheTNF-

αactivitybytheL929cellcrystalvioletstainingandthecellclimbingslides

werepreparedfordetectingtheNF-κBexpressioninmacrophagesby

immunocytochemistrymethod.ResultsBTMandresveratrolhadno

cytotoxicitytoL929cells.Afterprocessingbydifferentconcentrationsof

BTMandresveratrol,theTNF-αproductionlevelandthepositiverateof

NF-κBexpressioninmacrophagesshowedthedose-dependent

decrease.TheabilityofBTMforinhibitingtheTNF-αproductionleveland

NF-κBexpressinginmacrophageswasgreaterthanthatofresveratrol.

TheactivityofmacrophagesforproducingTNF-αandthepos-itivecell

rateofNF-κBwerenegativelycorrelatedwiththedrug

concentration,whiletheTNF-αactivitywaspositivelycorrelatedwiththe

positivecellrateofNF-κB.ConclusionBTMandresveratrolinthe

concentrationrangeof5-80μmol/LinhibitTNF-αproductionwitha

concentrationdependentmannerbyinhibitingNF-κBactivation.The

abilityofBTMforinhibitingTNF-αproductionandtheNF-κBactivation

inmacrophagesisgreaterthanthatofresveratrol.%目的：研究三甲氧基二苯

乙烯（BTM）对小鼠巨噬细胞经脂多糖(LPS)诱导后产生肿瘤坏死因子-α(TNF-α)

及细胞核因子-κB(NF-κB)活化的调控作用。方法培养RAW246.7小鼠巨噬细胞，

加入不同浓度的BTM或白藜芦醇，再加入LPS活化细胞，收集细胞上清液用

L929细胞结晶紫染色法检测TNF-α的活性，制作细胞爬片用免疫细胞化学法检

测巨噬细胞中NF-κB的表达。结果BTM和白藜芦醇本身对L929细胞无细胞毒

性；经不同浓度BTM和白藜芦醇处理后巨噬细胞产生TNF-α的水平及表达NF-

κB的阳性率呈剂量依赖性减少，BTM抑制巨噬细胞产生TNF-α及表达NF-κB