鸦胆子油口服乳液抑制肝癌发生的实验研究 .pdf

鸦胆子油口服乳液抑制肝癌发生的实验研究

李超英;陈文文;王楚盈;杨琳琳;迟源;张大方

【摘要】Objective:Thisstudyaimedtoinvestigatetheinhibitoryeffectsof

Bruceajavanicaoiloralemulsion(BJOOE)onprimarylivercancerinduced

bydiethylnitrosamine(DEN).Methods:Ratswererandomlydividedintothe

controlgroup,modelgroup,andBJOOEgroup.Ratsweregivenfree

accesstowater.DENwasadministeredintragastricallytoinduceliver

cancerinrats.Fiveweekslater,ratswereintragastricallyadministeredwith

BJOOEforfivetimesperweek.Theratswerekilledafter14weeks.

Abdominalaorticbloodsampleswerecollected.ThecontentsofALT,AST,

ALP,γ-GT,andAFPofserumweredetectedbyanautomaticbiochemical

analyzer.Theliverindex,spleenindex,thymusindex,andchangesinliver

cancernodulesofthesurfacewereobservedinrats.Changesinthe

numberoflivercancernodulesofthesurfaceweredetectedbyimaging.

Results:Comparedwiththecontrolgroup,theliverindex,spleenindex,and

numberofnodulesofthemodelgroupsignificantlyincreased,whereasthe

thymusindexsignificantlydecreased(P0.01).ThelevelsofALT,AFP,AST,

ALP,andγ-GTofseruminthemodelgroupweresignificantlyhigherthan

thoseinthecontrolgroup(P0.01).Comparedwiththemodelgroup,

BJOOEsignificantlyreducedtheliverindex,spleenindex,andnumberof

cancernodules,butincreasedthethymusindexintheliverofratswith

cancer(P0.01).ThelevelsofALT,AFP,AST,ALP,andγ-GTofseruminrats

withhepaticcarcinomasignificantlyimproved(P0.01orP0.05).

Conclusion:BJOOEcouldinhibitprimarylivercancer,andtheunderlying

mechanismsarecomplex.%目的：探讨鸦胆子油口服乳液对二乙基亚硝胺

（DEN）诱导的大鼠原发性肝癌的抑制作用。方法：将大鼠随机分为正常对照组、

模型对照组、鸦胆子油口服乳液组。采用自由饮水和灌胃给DEN的方法诱发大鼠

肝癌模型，诱发肝癌第5周开始灌胃鸦胆子油口服液，5次/周，14周后处死大鼠，

腹主动脉取血，全自动生化分析仪检测血清中ALT、AST、ALP、γ-GT和AFP的

含量，观察大鼠肝系数、脾系数、胸腺系数、肝脏表面的癌结节数的变化，并进行

影像学检查。结果：与正常对照组比较，模型对照组肝、脾指数和癌结节数明显增

加，胸腺指数明显降低（P0.01），血清中ALT、AFP、AS