48猪源致病性沙门氏菌耐药基因的分析.pdfVIP

,2006,37(1),65～70

ActaVeterinariaetZootechnicaSinica

12＊32321

,,,,,,

(1.,610064;2.,625014;

3.,610041)

:,、、411,30

,28(93.3%);、、

、、、,83.3%、

80%、80%、76.7%、60%、56.7%56.7%。25,,13

,GenBank(≥98.1%)。30

28(93.3%),sulⅠ、aph(3′)-Ⅱa、tetC、Cat1、tetAaadA1,

76.7%、60%、60%、43.3%、40%36.7%。(≥88%)。

:;;

+2

:S852.61:A:0366-6964(2006)01-0065-06

DetectionofAntimicrobialResistanceGenes

ofPathogenicSalmonellafromSwine

12＊323

MAMeng-gen,WANGong-ning,YUYong,LICheng-zhong,ZANGDong,

21

YANGYun-fei,LIUShi-gui

(1.CollegeofLifeScience,SichuanUniversity,Chengdu610064,China;

2.CollegeofAnimalScienceandTechnology,SichuanAgriculturalUniversity,Yaan625014,China;

3.SichuanProvincialOfficeofAnimalDiseasePreventionandControl,Chengdu610041,China)

Abstract:AntimicrobialMICsof30pathogenicsalmonellaisolatesfromswineweredeterminedby

usingplatedilutionmethod.11antibiotics(belongingtoaminoglycosides,tetracyclines,sulfona-

midesandchloramphenicols)wereused.Theresultsshowedthattwenty-eightstrains(93.3%)

ofthesesalmonellaisolateswereresistanttoatleastoneantimicrobialagent;resistancetothe

followingantibioticswascommon:tetracycline(83.3%),doxycycline(80%),sulfamethoxazole

(80%),sulfamethoxazoletimethoprim(76.7%),streptomycin(60%),kanamycin(56.7%)and

chloramphenicol(56.7%).25pairsofprimersweredesignedandantimicrobialresistancegenes

we