肌肉因子Irisin对脂肪环境下乳腺癌细胞影响的研究.pdf

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中文摘要······························································································III

Abstract································································································VI

1前言····································································································1

1.1乳腺癌的流行病学情况·················································································1

1.2肥胖和乳腺癌的发病关系··············································································1

1.3肥胖导致肿瘤微环境的改变···········································································1

1.4运动在乳腺癌防治及康复中应用的研究进展······················································3

1.5运动因子Irisin的研究进展及抗肿瘤作用···························································4

1.5.1运动因子Irisin的研究进展··················································································4

1.5.2运动介导的Irisin在慢性病预防中的作用································································5

1.5.3Irisin抗肿瘤作用的相关研究进展··········································································6

1.6推测Irisin可能通过影响CAAs及炎性因子抑制乳腺癌发展和侵袭··························7

2实验研究····························································································10

2.1Irisin对脂肪细胞共培养下乳腺癌发展及相关炎性因子的影响研究·························10

2.1.1MTT实验检测MDA-MB-231细胞活力验证Irisin对MDA-MB-231细胞增殖能力的影响··10

2.1.2Westernblot法检测NFκB蛋白表达情况，明确Irisin是否影响NFκB的表达··················18

2.1.3酶联免疫吸附法(ELISA)检测IL-6、肿瘤坏死因子-α、瘦素的水平，分析Irisin与肿瘤发生

发展过程中的炎性因子的相关性·······················································································25

2.1.4细胞迁移实验检测Irisin对MDA-MB-231细胞迁移和侵袭能力的影响··························31

2.2Irisin对乳腺癌相关因子影响的动物实验研究····················································36

2.2.1酶联免疫吸附法(ELISA)检测小鼠血清样本中相关因子表达水平·································36

3讨论·······

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