苦荞穗萌发的生理和QTL定位研究.pdf

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ABSTRACT······························································IV

1引言·······································································1

1.1苦荞的研究价值···························································1

1.2.穗萌发的产生与危害····················································1

1.3作物穗萌发研究进展·····················································2

1.3.1穗萌发抗性鉴定·····································································2

1.3.2种子的休眠············································································3

1.3.3种子活力与种子萌发·······························································4

1.3.4糖和蛋白与穗萌发··································································5

1.3.5淀粉和淀粉酶活性与穗萌发······················································6

1.3.6穗萌发QTL定位的研究···························································7

1.3.7荞麦中穗萌发研究概况····························································8

1.4研究目的和意义···························································8

1.5技术路线····································································9

2材料与方法······························································10

2.1试验材料··································································10

2.2试验仪器及试剂··························································11

2.2.1实验仪器·············································································11

2.2.2实验试剂·············································································11

2.2.3实验试剂的配置···································································12

2.3试验方法··································································13

2.3.1XJ-RILs田间穗萌发性状调查··················································13

2.3.2XJ-RILs群体种子萌发及发芽指标测定·······················

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