speciation方法汇总资料.doc

Thermo工厂文献提供方法

分析元素及形态

色谱条件

流动相

Hg2+MeHg

HypersilGOLD(150x4.6mm,5μm)

100ul进样量

60mMammoniumacetate,5%

methanol,0.01%2-mercaptoethanol

1.5ml/min等度分析

60mM醋酸铵（0.46%）

5%甲醇

0.01%2-巯基乙醇

800s前甲基汞和无机汞先后洗脱出峰。

Cr3+Cr6+

DionexIonPacòCS5A(250x2.0mm,5μm)

20ul进样量

0.35to1.2MHNO3

0.7ml/min等度分析

(1)AB(2)As3+(3)DMA(4)MMA(5)As5+

HamiltonPRPX100

20ul进样量

MobilePhaseCompositionPhaseA:10mMammoniumcarbonate

atpH8.9

PhaseB:20mMammoniumcarbonate

atpH8.9

GradientElutionProfile100%A:0-5.0minutes

100%Ato100%B:5.0-6.0minutes

100%B:6.0-22.0minutes

100%Bto100%A:22.0-23.0minutes

1ml/min梯度分析

(SeCy,SeMet,SeIVandSeVI)

PolymericAnionExchange

(250x4.6mm,10μm)

20ul进样量

BufferA20mMammoniumacetate:aceticacid,

pH4.7

BufferB200mMammoniumacetate:aceticacid,

pH4.7

Gradient

0min100%BufferA

20min100%BufferB

1.5ml/min梯度分析