氢化可松专着3443 hydrocortisone valerate3442.pdfVIP

3442Hydrocortisone/OfficialMonographsUSP35

200-mLvolumetricflask,addalcoholtovolume,andmix.PipetinthechromatogramobtainedfromtheStandardpreparation,

20mLoftheresultingsolutionintoaglass-stoppered,50-mLSS,andthesameratiointhechromatogramobtainedfromthe

conicalflask.Assaypreparation,SU.Calculatethequantity,inmg,offreehy-

Procedure—ToeachofthetwoflaskscontainingtheAssaydrocortisoneintheAssaypreparationtakenbytheformula:

preparationandtheStandardpreparation,respectively,andtoa

(100C)(S/S)

similarflaskcontaining20.0mLofalcoholtoprovidetheblank,US

add2.0mLofasolutionpreparedbydissolving50mgofblue

tetrazoliumin10mLofalcohol,andmix.ThentoeachflaskinwhichCistheconcentration,inmgpermL,ofUSPHydro-

cortisoneRSintheStandardpreparation;andSandSarethe

add4.0mLofa1in10solutionoftetramethylammoniumUS

hydroxideTSinalcohol,mix,allowtostandinthedarkfor90ratiosasdefinedabove.Theamountoffreehydrocortisoneis

minutes,add1.0mLofglacialaceticacid,mix,andproceedasnotmorethan6.7%ofthelabeledamountofhydrocortisone.

directedforProcedureunderAssayforSteroids〈