Glutathione Resin说明书用户手册.pdf

GlutathioneResinCat.No.L00206

TechnicalManualNo.TM0185Version

Index

1.ProductDescription

2.RelatedProducts

3.PurificationProcedure

4.Troubleshooting

5.OrderingInformation

1.ProductDescription

GenScriptGlutathioneResinisanaffinitychromatographymediumdesignedforeasy,one-steppurificationof

recombinantglutathioneS-transferase(GST)fusionproteinsandotherglutathionebindingproteinsexpressedinE.coli,

insectcellsandmammaliancells.TherecombinantGSTfusionproteinscanbepurifieddirectlyfrompre-treatedcell

lysateusingGlutathioneResin.Itistheexcellentchoiceforhighperformancepurifications.Table1liststhemain

characteristicsofGlutathioneResin.

Table1.CharacteristicsofGlutathioneResin

Resinvolume10mlsettledresin(20ml50%slurry)

LigandGlutathione

Dynamicbindingcapacity6mghorseliverGST(26kDa)/mlsettledresin

Matrixspherical4%cross-linkedagarose

Averageparticlesize90μm(45-165μm)

Storagesolution1XPBScontaining20%ethanol

Storagecondition2-8°C

Shelf12monthswhenstoredunopened

2.RelatedProducts

GenScriptalsoprovidestwokits,asthederivativeproductsofGlutathioneResin,tofacilitatetheexpressionand

purificationofGSTfusionproteins.

GSTFusionProteinPurificationKitCat.No.L00207

ProteinExpressionandPurificationKitCat.No.L00208

Table2.MainComponentsofGlutathioneResinanditsderivativeproducts

Components