基于网络药理学研究补阳还五汤治疗脊髓损伤的作用机制.docx

基于网络药理学研究补阳还五汤治疗脊髓损伤的作用机制

钟木森安忠诚樊光亚暴纪谦苏文硕董黎强

[摘要]?目的?基于網络药理学、分子对接验证补阳还五汤治疗脊髓损伤的作用机制。方法?通过TCMSP、SymMap、PubChem、Swiss?Target?Prediction数据库筛选补阳还五汤的活性成分和靶点。从OMIM、GeneCards、TTD、DrugBank数据库检索脊髓损伤的靶点。通过venny软件获取补阳还五汤与脊髓损伤的交集靶点。用Cytoscape软件构建补阳还五汤治疗脊髓损伤的活性成分-靶点网络。通过DAVID数据库对共同靶点进行基因本体论和京都基因与基因组百科全书富集分析，分子对接技术分析药物与靶点的结合能力。结果?共得到补阳还五汤中活性成分106个，作用靶点225个，脊髓损伤靶点1315个，药物-疾病交集靶点112个。补阳还五汤主要通过槲皮素、山柰酚、鞣花酸、木樨草素、常春藤皂苷元等药物活性成分治疗脊髓损伤。结论?补阳还五汤可通过多种信号通路达到治疗脊髓损伤的目的。

[关键词]?补阳还五汤；脊髓损伤；网络药理学；作用机制；分子对接

[中图分类号]?R683.2??????[文献标识码]?A??????[DOI]?10.3969/j.issn.1673-9701.2024.01.016

Mechanism?of?Buyang?Huanwu?decoction?in?the?treatment?of?spinal?cord?injury?based?on?network?pharmacology

ZHONG?Musen1，?AN?Zhongcheng2，?FAN?Guangya1，?BAO?Jiqian1，?SU?Wenshuo1，?DONG?Liqiang2

1.The?Second?School?of?Clinical?Medicine，?Zhejiang?Chinese?Medical?University，?Hangzhou?310053，?Zhejiang，?China;?????????2.Department?of?Orthopedics，?the?Second?Affiliated?Hospital?of?Zhejiang?Chinese?Medical?University，?Hangzhou?310005，?Zhejiang，?China

[Abstract]?Objective?To?verify?the?mechanism?of?Buyang?Huanwu?decoction?in?the?treatment?of?spinal?cord?injury?based?on?network?pharmacology?and?molecular?docking.?Methods?The?active?ingredients?and?targets?of?Buyang?Huanwu?decoction?were?screened?out?by?TCMSP，?SymMap，?PubChem?and?Swiss?Target?Prediction?databases.?Spinal?cord?injury?targets?were?retrieved?from?OMIM，?GeneCards，?TTD，?and?DrugBank?databases.?Through?venny?software，?the?intersection?target?of?Buyang?Huanwu?decoction?and?spinal?cord?injury?was?obtained.?The?active?ingredient-target?network?for?the?treatment?of?spinal?cord?injury?was?constructed?with?Cytoscape?software.?Gene?ontology?and?Kyoto?Encyclopedia?of?Genes?and?Genomes?enrichment?analysis?of?common?targets?were?carried?out?by?DAVID，?and?the?binding?ability?of?drugs?and?targets?was?analyzed?by?molecular?docking?technology.?Results?A?total?of?106?active?ingredients?and?225?targets?of?Buyang?Huanwu?decoction，?1315?targets?of?spinal?cord?injury?and?112?targets?of