基于数据非依赖性采集定量蛋白质组学分析的原发性干燥综合征潜在唾液生物标志物研究.pdfVIP

ResearchArticleChinMedSciJ

2024;39(1):19-28

doi:10.24920/004338

Data-IndependentAcquisition-BasedQuantitativeProteomic

AnalysisRevealsPotentialSalivaryBiomarkersofPrimary

SjogrensSyndrome

Yi-ChaoTian,Chun-LanGuo,ZhenLil,XinYou²,Xiao-YanLiu,Jin-MeiSu²,Si-JiaZhao,

YueMu,WeiSun3*,QianLi1*

1.DepartmentofStomatology,PekingUnionMedicalCollegeHospital,ChineseAcademyofMedicalSci-

encesPekingUnionMedicalCollege,Beijing100730,China.

2.DepartmentofRheumatologyandClinicalImmunology,PekingUnionMedicalCollegeHospital,Chi-

neseAcademyofMedicalSciencesPekingUnionMedicalCollege,Beijing100730,China.

3.ProteomicsCenter,CoreFacilityofInstrument,InstituteofBasicMedicalSciencesofChineseAcademy

ofMedicalSciencesSchoolofBasicMedicinePekingUnionMedicalCollege,Bejing100730,China.

ABSTRACT

ObjectiveAsprimarySjogrenssyndrome(psS)primarilyaffectsthesalivaryglands,salivacanserveas

anindicatoroftheglandspathophysiologyandthediseasesstatus.Thisstudyaimstoillustratethesalivary

proteomicprofilesofpSSpatientsandidentifypotentialcandidatebiomarkersfordiagnosis.

MethodsThediscoverysetcontained49samples(24frompSSand25fromage-andgender-matchedhealthy

controls[HCs)andthevalidationsetincluded25samples(12frompSSand13fromHCs).Totally36pSS

patientsand38HCswerecentrallyrandomizedintothediscoverysetortothevalidationsetata2:1ratio.

UnstimulatedwholesalivasamplesfrompSSpatientsandHCswereanalyzedusingadata-independentacquisition

(DIA)strateg