s12870-025-06172-y能源环保科技报告.pdf

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Jingetal.BMCPlantBiology(2025)25:267BMCPlantBiology

/10.1186/s12870-025-06172-y

RESEARCHOpenAccess

Integratedphysiologicalandtranscriptomic

analysesrevealthatcellwallbiosynthesis

andexpansionplayanimportantroleinthe

*

regulationofplantheightinalfalfa

11*1111

Fang

Jing,Shang-Li

Shi,Wen-Juan

Kang,Bei

Wu,Bao-Fu

LuandJian

Guan

Abstract

BackgroundAlfalfa(MedicagosativaL.)isahigh-quality,high-proteinforage,andtheimprovementandbreeding

ofkeytraitsareimportantforenhancingtheproductivityofalfalfa.Plantheightisanimportanttraitthataffectscrop

yield,anditsregulatorynetworkmechanismhasbeenwidelyreportedinmodelplants,however,therearefewer

studiesonthedevelopmentalregulatoryofplantheightinalfalfa.

ResultsInthisstudy,wescreenedtall(WL525HQ)andshort(WL343HQ)alfalfamaterialsthroughfieldexperiments

andanalyzedtheregulatorymechanismofplantheightbasedonthemultidimensionaljointanalysisofphenotype,

cell,physiology,andmolecularbiology.Theresultsshowedthatinternodelengthwasanimportantfactor

determiningplantheightinalfalfa,andcellsizeaffectedtheinternodeelongationtoacertainextent,whereas

cellsizewaslimitedbycellwall.Moreover,changesincellwallcomponentsplayanimportantroleincellwall

expansion,especiallyligninsynthesis.Transcriptomeanalysisshowedthatthehighexpressionofhydrolaseactivity

inT1(initiationgrowthperiod)facilitat

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