细胞通讯信号通路组成及内外信号分子差异.pdfVIP

Cellcommunication

信号通路的组成

Signalmoleculeextracellularorintracellularmolecularthatcues(暗示)the

respondsofacelltothebehaviorofothercellorobjectsinenvironment

Receptorproteinbindsthesignalmolecular

Intracellularsignalingproteindistributethesignaltoappropriatepartofcell

Targetproteinwhicharealteredwhenthepathwayisactiveandchangethebehavior

ofthecell

Signalmolecule

1)smallpeptide2)aminoacid3)nucleotide4)steroid

5)retinoid(类维生素A)6）fattyacidderivatives7)NOCO2

NO信号传导途径

1.acetylcholine(乙酰胆碱)bindstothesurfaceofcell

2.cytosolicca2+concentrationrise

3.ca2+activatesNOsynthaseandsynthesizeNOfromArg

4.NOdiffuseacrossmembraneenterthesmoothmusclecell

5.NOactivateguanylcyclaseandsynthesizeC-GMP

6.c-GMPleadstodecreaseincytosolicCa2+concentrationandcauserelaxation

ofmusclecellandbloodvessel(血管)

细胞内外信号的区别

Extracellularintracellular

Hydrophobichydrophilic

Lastingshorterlastinglonger

Outofcellincell

Srchomology2(SH2)domainandphosphotyrosine-binding(PTB)domain

BindstophosphorylationTyrinaparticularpeptidesequenceonactivatedreceptor

orintracellularsignalingprotein

Srchomology3(SH3)domain

Bindstoashortpro-richaminoacidsequence

PHdomainbindtothechargeheadgroupsofspecificphosphorylatedinositol(肌

糖)phospholipid

Featureofsignalingpathway

1.specificitysignalmoleculefitsbindingsiteonitscomplementaryreceptor

2.amplificationwhenenzymeactivateenzyme,increaseveryrapidly

3.desensitization/adaptaionreceptoractivationtriggerafeedbackcircuit

thatshutsofftherec