Rh系统简单特异性自身抗体血清学鉴定与分析.pdfVIP

临床血液学杂志2024年

586ClinHematol(China)37卷8期

Rh系统简单特异性自身抗体血清学鉴定与分析

张刘仟1马玲?邵雷？减炫月1尤建国！

［摘要］目的：通过2例Rh系统简单特异性自身抗-e抗体案例的鉴定和分析，了解简单特异性自身抗体的

血清学特征。方法：采用常规血清学方法对患者进行ABO、Rh表型分型，直接抗人球蛋白试验对患者血清及放

散液中抗体进行检测；进一步采用不同表型细胞对患者血清吸收后进行抗体鉴定，并对吸收后细胞再次放散检

测。结果：患者均为e抗原阳性，血清和放散液中表现出抗-e抗体格局，该抗体只能被e抗原阳性细胞吸收，而不

能被e抗原阴性细胞吸收。结论：该2例抗体为简单特异性自身抗e抗体，不能被e抗原阴性细胞吸收，与类抗

体特征存在区别。

［关键词］简单特异性自身抗体；抗体鉴定；抗-e

D0l:10.13201/j.issn.1004-2806.2024.08.013

[中图分类号]R457.1[文献标志码]］A

SerologicalidentificationandanalysisofsimpleRh-specificautoantibodies

ZHANGLiuqianlMALingSHAOLei?ZANGXuanyuelYOUJianguo!

(DepartmentofBloodTransfusion,HuaianFirstPeoplesHospital,Huaian,223300,China;

2ResearchLaboratoryforTransfusion,JiangsuProvinceBloodCenter)

Correspondingauthor:YOUJianguo,E-mail:youjg1968@163.com

AbstractObjective:Tounderstandtheserologicalcharacteristicofsimplespecificautoantibodiesbystudies

of2patientswhoproducedsimplespecificautoanti-eantibodies.Methods:ABO,Rhphenotyping,DATandanti-

bodyidentificationwereconductedbyroutineserologicalmethods.Antibodyidentificationwasdoneusingdifferent

phenotypeRBCswiththeelutingsolutionandabsorbedserum.Results:Both2patientswereepositive.Theanti-

bodiesinserumandelutingsolutionwereidentifiedasanti-ewhichcouldnotbeabsorbedbyenegativeRBCs.

Conclusion:Thesetwoantibodiesweresimplespecificautoanti-e.ItcannotbeabsorbedbyenegativeRBCswhich

distinguishwithmimickingantibody.

Keywordssimplespecificantibody;antibodyidentification;anti-eantibody

临床输血前常规对患者开展不规则抗体筛查，肺炎呼吸衰竭。人院检测血红蛋白45g/L，HCT

可以降低免疫性因素导致的输血不