BODIPY-TR-Cadaverine-solution-生命科学试剂-MCE.pdfVIP

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BODIPYTRCadaverine(solution)

Cat.No.:HY-DY1076

CASNo.:217190-24-4

分⼦式:C₂₆H₂₈BClF₂N₄O₂S

分⼦量:544.85

作⽤靶点:FluorescentDye

作⽤通路:Others

储存⽅式:Pleasestoretheproductundertherecommendedconditionsin

theCertificateofAnalysis.

BIOLOGICALACTIVITY

⽣物活性BODIPYTRCadaverine(solution)是⼀种cadaverine衍⽣物，是⼀种红⾊荧光染料。BODIPYTRCadaverine

可⽤于⼀种⾼灵敏度、⾼强度的荧光置换试验，它能与天然LPS强烈结合，特异性识别脂质A，并被显⽰对脂

质A有亲和⼒的化合物竞争性地置换。

溶剂及浓度：DMSO：2mM

体外研究BODIPYTRCadaverineassay[1](toquantifyautophagyincells):

Cellswereincubatedwith125nMBODIPYTRCadaverinefor10minutes,washedfourtimeswithPBSand

lysedin10mMTris-ClpH8,0.1%TritonX-100.FluorescencelevelswerereadinaSPECTRAmax

fluorimeter(MolecularDevices)(λex=588nm,λem=616nm).Fluorescencelevelswerenormalizedforcell

numberbyadding0.2mMethidiumbromideandreadingDNAfluorescence(λex=530nm,λem=590nm)[1].

REFERENCES

[1].CarreiraRS,etal.CyclophilinDisrequiredformitochond