研究生英文文献汇报演示文稿.pptVIP

研究生英文文献汇报演示文稿第一页，共20页。

第二页，共20页。

Keywordsscutellarin;scutellarein;UGT;enzymeinhibition.第三页，共20页。

Contents1.Introduction2.Experimentalsection3.Resultsanddiscussion4.Conclusion第四页，共20页。

1.Introduction第五页，共20页。

Intherecentyears,anotherimportantdrugmetabolizingenzymeUDP-glucuronosyltransferase(UGT)hasbeendemonstratedtoexhibitsignificantcontributiontowardsthemetabolismofclinicaldrugsandherbalcomponents,andmoreandmoreattentionhasbeengiventothisenzymes(MalikandBlack,2012;Lietal.,2012).UGTshavebeendemonstratedtobeinvolvedinthemetaboliceliminationofmanyimportantendogenoussubstances,suchasbilirubin,bileacidandestradiol(Erichsenetal.,2010).第六页，共20页。

Guoetal.showeddeglycosylationprocessofliquiritinstronglyenhancedtheinhibitorycapabilitytowardsUGTs(2012).Huangetal.showedstronginhibitionofglycyrrhetinicacidtowardsUGT1A3and2B7(2012).Liuetal.alsousedinvitroincubationsystemtofindthestronginhibitionofdeoxyschizandrinandschisantherinAtowardsUGT1A3(2012).第七页，共20页。

Chemicalsandreagents.Scutellarin,Scutellarein,4-MU,Tris-HCl,7-hydroxycoumarin,UDPGA(缓冲盐)，重组UGTs亚型（UGT1A1，UGT1A6，UGT1A9，UGT2B7），HPLCreagents.2.Experimentalsection第八页，共20页。

Incubationandanalysismethodsforinhibitionevaluation.After5minpre-incubationat37℃,theUDPGAwasaddedinthemixturetoinitiatethereaction.Theincubationtimewas120minforUGT1A1andUGT2B7,30min,orUGT1A6andUGT1A9,respectively.Thereactionswereterminatedbyadding100mLacetonitrilewith7-hydroxycoumarin(100mM)asinternalstandard.Themixturewascentrifugedat20000×gfor10min,HPLCanalysis.第九页，共20页。

Datafittingfordeterminationofinhibitiontypeandparameters(Ki).Thereactionvelocitywasdeterminedusingvariousconcentrationsof4-MUandscutellarein.ThedatawerefittedusingDixonplotandLineweaver–Burkdoublereciprocalplot.Thesecondplotwiththeslo