有参不比对转录组分析流程：Salmon定量与差异表达.pptxVIP

12转录组有参分析Salmon流程易生信，毕生缘；培训。

易生信四种常规的转录组分析策略易生信，毕生缘；培训。2Stringtie TrinotateTrinotate (d)不基于比对， 直接定量流程 Reference transcriptHISAT2 Reads Sailfish/kallisto /Salmon-SMEM /Salmon-QuasiAlignment-free isoform quantification counting

易生信有参不比对转录组分析流程易生信，毕生缘；培训。3Sequencing RNA-seq reads(2x150bp)Genequantification Salmon (cDNA)Differentialexpression DESeq2 ImageGPVisualization Gene annotation(.gtffile)(可选)Rawsequence data (.fastqfi