留兰香挥发油提取及其体外抗植物真菌研究.pdfVIP

中国农学通报 2011,27(7):262-265 Chinese Agricultural Science Bulletin 留兰香挥发油提取及其体外抗植物真菌研究 邹正耀，张红英，王学兵，张元元，崔保安 （河南省动物性食品安全重点实验室，河南农业大学牧医工程学院，郑州450002 ） 摘 要：为探讨留兰香挥发油对植物真菌的体外抑制效果，应用水中蒸馏法提取留兰香挥发油并配制成 不同浓度的挥发油乳液，用试管二倍稀释法对选取的3 种植物真菌：玉米大斑病菌、番茄灰霉病菌、辣椒 疫霉病菌进行体外抑菌试验。结果表明：留兰香挥发油乳液对玉米大斑病菌在24 h 内有一定的抑菌效 果，而在36 h 、48 h 观察时抑菌效果减弱至消失；对番茄灰霉病菌有较好的抑菌杀菌效果，在高浓度时有 杀菌效果，低浓度时有抑菌效果；对辣椒疫霉病菌则没有抑菌效果。以上结果表明，留兰香挥发油乳液 对部分植物真菌具有良好地抑制作用。 关键词：留兰香挥发油；植物真菌；玉米大斑病菌；番茄灰霉病菌；辣椒疫霉菌 中图分类号：S432.2+6 文献标志码：A 论文编号：2010-2533 ExtractionandAntimicrobialActivityofSpearmintOilonPlantEumyceteinVitro Zou Zhengyao, Zhang Hongying, Wang Xuebing, Zhang Yuanyuan, Cui Bao ’an (CollegeofAnimalHusbandryandVeterinary,HenanAgriculturalUniversity, Zhengzhou 450002) Abstract:To study the inhibitory activity on plant eumycete in vitro of spearmint oil, which was extracted by steam distillation method and prepared to different concentration. 3 kinds of plant eumycete: Helminthosporium turcicum, Botrytis cinerea and Phytophthora capsici were chosen for test by using double dilution of test tube method. The results showed that: Spearmint oil exhibited some inhibitory activity on Helminthosporium turcicum at 24 h. But the activities weaken and vanished at 36 h and 48 h. It exhibited fine inhibitory activity against Botrytis cinerea. It also exhibited little inhibitory activity on Phytophthora capsici. Spearmint oil exhibited fine inhibitory activity against some plant eumycete. Keywords:spearmint oil; plant eumycete; Helminthosporium turcicum; Botrytis cnerea; Phytophthora capsici 0 引言 （Asp ergillus ochraceus）和稻恶苗病菌 （Fusarium [2] [3] 留兰香（Mentha sp i