血浆miRNA表达谱与肺癌预后关系研究.pptVIP

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2017-09-29 发布于北京
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血浆miRNA表达谱与肺癌预后关系研究.ppt

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In lung cancer, it has been shown that miRNA expression profiles and specific miRNAs in lung tissue are correlated with disease prognosis and clinical outcome Cancer Cell. 2008;13:48-57. miRNAs Exist in Serum? Circulating microRNAs as stable blood-based markers for cancer detection PNAS, 2008 Jul Why Serum Biomarkers are Important? Easy to get (non-invasive) Repeatable In high risk population (cancer screening) Solexa Whole Genome Sequencing Subjects and Assays Serum RNA Extraction Efficiency and the Reliability of qRT-PCR Assay Copies of Solexa Sequencing and qRT-PCR Expression of the 11 miRNAs (30 LS vs 30 SS) Risk Score Analysis of 303 NSCLC Patients Risk Score and NSCLC Survival Stepwise Cox Proportional Hazards Analyses on NSCLC Survival Conclusions Serum miRNA signature can be also used to predict the subgroup of NSCLC patients with poor prognosis (short survival). Thanks! 沈洪兵南京医科大学公共卫生学院 Tel. (Fax.): 86-25Email: hbshen@njmu.edu.cn 血浆miRNA表达谱与肺癌预后关系研究 (1) In the nucleus, pri-miRNAs processed by Drosha as pre-miRNAs; (2) pre-miRNAs exported by Exportin-5 into cytoplasm; (3) Processed by Dicer as mature miRNAs; (4) Perfect complement with 3’UTR: mRNAs cleavage; in-perfect complement: translational repression. Nat. Rev. Genet. 5(7):522-31,2004 MicroRNAs and Cancer miRNA as Novel Important Tumor Suppresser Genes and Oncogenes The discovery of microRNAs (miRNAs) has opened new avenues for cancer diagnosis and prediction of treatment response Nature Reviews Cancer 6:259-269, 2006 Nature 435(7043):834-838,2005 miRNA expression profiles can classify human cancers miRNA Profiling May Provide More Accurate Classification of Cancer Subtypes than the Expression Profiles of Protein-coding Genes Cancer Cell. 2006;9:189-98. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases Cell Res. 2008 Oct The discovery of stable serum miRNAs Chen et al. 2007 Cell Res. miRNAs (21-23nt) we