现代分析技术实验.pdf

现代分析实验与技术 编撰人：付绍平 杨红 大连轻工业学院－生物与食品工程学院 生物分析教研组 2006 年 12 月 仪器分析是一门实践性、技术性很强的综合性学科，是检验各种原料、分 析产品质量的重要工具。基于《新编仪器分析》的教学内容，考虑到本校教学 仪器实际情况，我们编著写了本实验教材，适合本科生、研究生仪器分析实验 教学使用。 本实验教材分成六章，由光谱分析方法（紫外吸收光谱法、红外吸收光谱 法、原子吸收光谱法、荧光光谱法）和分离分析方法（气相色谱法、液相色谱 法）组成。通过实验，我们可以了解各种分析仪器的基本结构和基本原理，掌 握各种分析方法的基本原理，掌握仪器分析实验的基本操作技能，加深理解并 掌握仪器分析的基本知识和原理，并进一步培养分析问题和解决问题的能力。 本实验讲义付绍平、杨红老师编写，欢迎就错误和不当之处提出宝贵意见。 2006 年12 月 实验基本要求： 1. 做好实验预习（明确实验目的、任务、有关原理、操作的主要步骤、注意事项，并于实 验前写好实验预习报告）； 2. 实验中要手脑并用，细心记录实验现象和数据，严格遵守操作规程及注意事项，自觉遵 守实验室各项规章制度； 3. 实验的原始记录经指导教师签阅后妥善保管备查，认真撰写实验报告。 目 录 实验一 紫外吸收光谱法测定银黄片中黄芩苷和绿原酸的含量 1.1 目的与要求···································································································1 1.2 实验原理与方法提要···················································································1 1.2.1 光吸收基本定律－Lambert-Beer 定律············································· ·1 1.2.2 吸光度的加和性··················································································2 1.2.3 方法提要······························································································2 1.3 仪器与试剂···································································································3 1.4 操作步骤·······································································································3 1.5 fUV755B基本操作··························································································4 1.6 思考与讨论···································································································4 实验二 荧光法测定硫酸喹啉的含