茉莉酸诱导棉花幼苗抗虫性对棉铃虫相对生长率的影响.docVIP

  茉莉酸诱导棉花幼苗抗虫性对棉铃虫相对 生长率的影响# 5 10 15 20 25 30 35 40 45  摘要：【目的】研究不同浓度茉莉酸诱导皖棉 16# 抗虫相关酶活性的浓度依赖性和系统诱 导，以及某一特定浓度茉莉酸诱导抗虫性的滞后期及其对棉铃虫相对生长率和质量增加的影 响, 为农林害虫的综合防治提供理论依据。【方法】以皖棉 16# 为材料，分别以 0.1, 1.0 和 10 mmol/L 茉莉酸处理 6 叶期棉花幼苗叶片，研究不同浓度茉莉酸对棉花幼苗抗虫相关酶活 性的影响；以 1.0 mmol/L 茉莉酸处理 6 叶期棉花幼苗，研究其对棉花幼苗抗虫性诱导的时 效性及其对棉铃虫相对生长率和质量增加的影响。【结果】0.1 mmol/L 茉莉酸对棉花幼苗 多酚氧化酶(PPO)、过氧化物酶 (POD) 和苯丙氨酸解氨酶 (PAL) 的诱导作用不明显，但能 诱导处理叶脂氧合酶 (LOX) 活性显著升高；1.0 mmol/L 茉莉酸能诱导棉花幼苗 PPO 和 PAL 活性局部升高和系统升高，但只能诱导 POD 系统升高和 LOX 活性局部升高；10 mmol/L 茉莉酸诱导棉花幼苗 PPO 活性局部升高和系统升高，诱导 PAL 活性系统升高， 诱导 LOX 活性系统下降，对 POD 活性的影响不明显。分析表明，1.0 mmol/L 茉莉酸是 6 叶期棉花幼苗抗虫相关酶诱导的最佳浓度，依酶而异，诱导效应可持续 4-8 天，但处理后第 6 d 的叶对棉铃虫相对生长率和质量增加量的抑制作用最明显。【结论】茉莉酸对棉花幼苗 抗虫相关酶的诱导存在浓度依赖性和滞后期, 诱导抗性明显抑制棉铃虫的相对生长率和质 量增加。1.0 mM 茉莉酸是诱导 6 叶期皖棉 16# 幼苗抗虫性的最佳浓度，抗虫性的滞后期可 达 4-8 天。 关键词：诱导防御；棉花；棉铃虫；抗消化酶；相对生长率；害虫控制 中图分类号：S433 Jasmonic acid-mediated plant defense in cotton seedlings against the relative growth rate of Helicoverpa Armigera YANG Shiyong1, SONG Fenfang1, XIW Jianchun2 (1. School of Life Sciences, Anhui Normal University; 2. School of Environment Science and Engineering, Anhui Normal University) Abstract: IObjective】The concentration dependency and systemic induction in Wanmian 16# (Gossypium hirsutum, L) by different concentrations of jasmonic acid (JA), as well as the time effect after JA application on the induced pest defense and its effect on the relative growth rate and mass gain of Helicoverpa armigera was investigated, thereby supplying theoretical fundamentals to the integrated pest management of agricultural and forestry pests. 【Method】0.1, 1.0 and 10 mmol/L jasmonic acid were used to investigate their effects on the induction of pest defense enzyme activities in six-foliage seedlings of cotton plant Wanmian 16 #. 1.0 mmol/L jasmonic acid was used to study the time effect of JA-induced pest defense in cotton seedlings as well as its effect on the relative growth rate and mass gain of H. armigera. 【Result】0.1 mmol/L JA had insignificant effect on the induction of polyp