PLLA_PCL复合纳米纤维支架的电化学协同修饰及生物相容性研究.docVIP

  PLLA/PCL 复合纳米纤维支架的电化学 协同修饰及生物相容性研究# 曹利军1，冯炜1，仇可新2，胡金伟1，程晓1，何创龙1** 5 10 15 20 25 30 35 40 （1. 东华大学化学化工与生物工程学院，上海 201620； 2. 材料科学与工程学院，上海 201620） 摘要：选用牛血清白蛋白（BSA）和羟基磷灰石(HA),通过电化学沉积技术对制备的 PLLA/PCL 复合纳米纤维支架进行表面修饰，以提高支架的生物相容性。通过扫描电镜（SEM）、傅里 叶红外光谱（FTIR）以及 X-射线衍射（XRD）等手段对沉积物的形貌及组成进行表征，并研 究了矿化支架的细胞相容性。SEM 观察发现，由于 BSA 的加入，沉积的晶体由针状结构转变 为片状结构。FTIR 结果表明复合涂层中存在磷酸钙盐成分和蛋白质的氨基特征峰。XRD 分析 表明沉积的晶体成分主要是羟基磷灰石（HA）。体外实验表明，大鼠前成骨细胞（MC3T3-E1） 在矿化支架上具有良好的黏附、增殖和分化能力。 关键词：生物医学工程；电化学沉积；BSA/HA；协同矿化 中图分类号：R318.08 Electrochemical co-operation surface modification for PLLA/PCL hybrid microporous and nanofibrous scaffolds and biocompatibility research CAO Lijun1, FENG Wei1, QIU Kexin2, HU Jinwei1, CHENG Xiao1, HE Chuanglong1 (1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, ShangHai 201620; 2. College of Materials Science and Engineering, Donghua University, ShangHai 201620) Abstract: A high quality BSA/HA coating can be produced on PLLA/PCL hybrid scaffolds within a couple of hours by electrochemical deposition technology. The morphology and composition of the resulting coating were analyzed by SEM, FTIR and XRD. The biocompatibility of the mineralized scaffolds were valuated by using mouse pre-osteoblast MC3T3-E1cell. The morphology of the crystals was needle-like structure and similar to the topography of hydroxyapatite (HA) in nature bone tissue, while the morphology of the crystals was changed from needle-like to leaf-like when BSA and HA were co-deposited on the surface of scaffolds. The results of FTIR spectra showed the existence of HA and BSA in mineralized PLLA/PCL hybrid scaffolds, and the XRD results suggested that the component of the crystals coatings deposited at a constant voltage (3 V) and temperature (37 ℃) were almost HA containing small amount of DCPD. The biological response of MC3T3-E1 cells on the mineralized scaffolds containing BSA was superior to their counterparts (without BSA) in terms of higher proliferation, well-spread morpholog