拟南芥耐硒突变体的鉴定及基因定位.docVIP

  拟南芥耐硒突变体的鉴定及基因定位# 陈大清1，钟育海2，李应生2，苏爱国2，李亚男3** （1. 仲恺农业工程学院生命科学学院，广州 510225； 5 10 2. 长江大学生命科学学院，荆州 434023； 3. 长江大学农学院，荆州 434023） 摘要：在硒盐胁迫下通过根弯曲特性筛选获得耐硒突变体，并对该突变体从 M3- M5 代进行 硒耐受稳定性测试，结果表明突变体可以稳定遗传。以突变体为母本与野生型（Ler）杂交 获得基因型完全杂合的 F1 代；自交产生的 F2 代硒敏感：硒耐受的分离比为 3：1，这表明 该突变体是隐性单基因遗传。从 F2 代选出 132 株具有耐硒特性的植株作为图位克隆的群体， 应用 SSLP 和 In/Del 分子标记进行基因定位，通过分析目的基因与各分子标记之间的连锁关 系，发现耐硒突变体基因位于第 I 号染色体 F12P19 和 NGA111 的分子标记之间。 关键词：拟南芥；硒盐胁迫；突变体；基因定位 中图分类号：Q945.1 15 Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis Chen Daqing1, Zhong Yuhai2, Li Yingsheng2, Su Aiguo2, Li Yanan3 (1. School of Life Science,Zhongkai University of Agriculture and Engineering, Guangzhou, 20 25 30 510225; 2. School of Life Science,Yangtze University, Jingzhou, 434023; 3. School ofAgronomy,Yangtze University, Jingzhou, 434023) Abstract: A selenate-torlerance arabidopsis mutant was obtained by using the root-bending trait under selenate stress.The mutant identification of selenate resistant stability were detected from generation M3 to M5 continuously,It was conformed that the selenate resistant characteristics could be steady inheritance.Then this mutant was crossed with the wild-type Ler.and F1 generation were created with heterozygous type completely.Tne F2 crossed by theirself were expressed the separation ratio of 3:1 in response of sensitive selenium to resistent selenium,It were suggested that this mutant was a monogenic recessive mutation. Map-based cloning population were composed of the 132 selenate-tolerance seedlings in F2 generation. Using SSLP and In/Del based on PCR to preliminary chromosomal location. Through linkage analysis, it’s indicated that this mutant gene was located on the chromosome I, which was mapped between molecular marker F12P19 and NGA111. Key words: Arabidopsis thaliana; Selenate stress ;mutant;Gene Lacation 35 0 引言 微量元素硒(Se)不仅是人、动物和微生物的必需营养元素,也是植物生长发育的有益元素 [1,2] 。植物中的硒含量变幅从几 μg.kg-1 干重到几千 mg.kg-1 干重,超积累型植物可高达几千 mg.kg-1 干重[3 ],这为研究植物硒代谢提供了潜在的遗传资源。关于