石菖蒲活性成分配伍对鹅膏蕈氨酸致痴呆大鼠学习记忆功能的影响.docVIP

  石菖蒲活性成分配伍对鹅膏蕈氨酸致痴呆 大鼠学习记忆功能的影响# 田会娟1，陈云波1，魏刚2，林雅萍1，刘玉1，程淑意1， 5 10 15 20 25 30 35 王奇1，梁伟雄1，陈东风3** （1. 广州中医药大学临床药理研究所 DME 中心，广州 510405； 2. 广州中医药大学新药开发研究市中心，广州 510006； 3. 广州中医药大学基础医学院，广州 510006） 摘要：目的：观察石菖蒲两种活性成分单体β-细辛醚、丁香酚按 4:1 比例配伍不同剂量对 鹅膏蕈氨酸（IBO）致阿尔茨海默病模型大鼠学习记忆功能的影响。方法:SD 大鼠 119 只， 随机分为 6 组，即假手术组、模型组、安理申阳性药物对照组、低、中、高剂量配伍组。 假手术组给予双侧 Meynert 基底核各注射 1 微升生理盐水，其余各组均给于相同部位等量注 射 IBO 溶液制备学习障碍模型。正常组及模型组给予灌服相同体积饮用水，其余各组均给 予灌服相应的治疗药液。灌胃 28 天后开始通过 Morris 水迷宫测试小鼠行为学变化及学习 记忆能力，以逃避潜伏期和游泳路程作为观测指标。结果：与假手术组比较，模型组的逃避 潜伏期和游泳路程均明显延长；与模型组比较，安理申组及高剂量配伍组的逃避潜伏期和游 泳路程均明显缩短(P0.01，P0.05)。结论：石菖蒲中有效成分配伍高剂量对 ibo 导致的大 鼠学习记忆障碍有改善作用。 关键词：阿尔茨海默病；鹅膏蕈氨酸；配伍；学习记忆 中图分类号：R 749.1+6 Influence of various doses of the combinations of β-asarone and eugenol in Acorus Tatarinowii on Learning-Memory Ability in Dementia Model Rats Induced by Ibotenic Acid TIAN Huijuan1, CHEN Yunbo1, WEI Gang2, LIN Yaping1, LIU Yu1, CHENG Shuyi1, WANG Qi1, LIANG Weixiong1, CHEN Dongfeng3 (1. DME Center, Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; 2. New Drug Research Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; 3. School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, China) Abstract: Object: To observe the influence of various doses of the combination of β-asarone and eugenol with the ratio of 4:1 in Acorus Tatarinowii on learning-memory ability in Alzheimers Disease (AD) model rats induced by Ibotenic Acid. Methods: 119 rats were randomly divided into 6 groups: sham operation group, Scop model group, Donepezil hydrochloride group, and low dose，middle dose and high dose of the combination of β-asarone and eugenol. The rats in sham operation group were receiving injection of 1μl saline solution into each side of bilateral NBM. The other groups received the same amount of ibotenic acid in the same region. Rats were perfused with same volume of water or 40 drugs respectively for 28 day