MRSA感染的早期识别和快速诊断.ppt

MRSA快速确认试验：Real-time PCR Three FDA cleared products BD GeneOhm (Infectio Diagnostic) Cepheid GeneXpert LightCycler(R) MRSA advanced test Samples type is direct nasal swab Detects gene sequence at the junction of the SCCmec and orfX gene Kit based product that can be used with minimal specialized training Clinical Infectious Diseases 2011;52(S4):S361–S366 Clinical Infectious Diseases 2011;52(S4):S361–S366 MRSA来源分析： 菌株分型 MRSA is a clonal organism Very useful for epidemiology Strain types between patients Monitor trends in types Track seasonal outbreaks Database of samples for long term studies Trace infection source Patient-Patient / Patient-Caregiver Determine if HA or CA MRSA 美国MRSA Strain Types Large study by CDC in 2003 Sma I digested bacterial DNA ~1000 MRSA isolates Determine CA vs. HA strains Eight major strain types in US CA USA 300 and 400 Resistant to beta-lactams and erythromycin USA 100 most common type (44%) Multiresistant USA 700 both HA and CA 各种MRSA菌株分型方法的分辨力比较 常用菌株分型方法的实用性比较 谢 谢 在组设置中可使用此模板作为演示培训材料的起始文件。 节 右键单击幻灯片可添加节。 节可以帮助您组织幻灯片或促进多个作者之间的协作。 备注 使用“备注”节传递备注或为观众提供更多详细信息。演示过程中，可在演示文稿视图中查看这些备注。 请记住字体大小（这对于可访问性、可见性、录像和联机制作都非常重要） 协调的色彩 要特别注意图形、图表和文本框。 请考虑与会者将以黑白或灰色调打印。请运行测试打印，以确保当以纯黑白和灰色调打印时，颜色正常。 图形、表格和图表 保持简约风格：如果可能，请使用一致的、不分散人注意力的样式和颜色。 标记所有图表和表格。 * * 06年全球著名的医学杂志《柳叶刀〉上发表了一篇文章，疾呼耐药葡萄球菌在全球范围内快速蔓延，已经成为全球共同面对的公共卫生问题。 这张图反映了世界各地所报道的MRSA的发生率：不同的颜色显示不同的发生率，亚洲的香港、新加坡、日本等都已超过了50%。 中国的CHINET报道，2005年中国的MRSA、MRCNS的发生率分别为69%和82% * The Burden of MRSA The burdens of health care–associated MRSA are increased hospitalization, increased cost, and increased mortality. Because of the rising rates of MRSA—and the associated morbidity, cost, and mortality—it is clear that a better solution for managing MRSA infections is needed.1 Cost Across studies, the higher cost for treating MRSA infections reflected the added cost of therapy, extra days spent in the hospital, and the cost of patient isolation procedures. In MRSA bacteremia, Abramson observed a 2.8-fo