鄱阳湖黄颡鱼染色体核型分析及进化地位探讨.pdfVIP

江西农业大学学报 20 12 ，34 (6):1222 － 1225 http :/ / xuebao． jxau． edu． cn Acta Agriculturae Universitatis Jiangxiensis E － mail :ndxb7775@ sina． com 鄱阳湖黄颡鱼染色体核型分析 及进化地位探讨 1 1 2 1 1 毛慧玲 ，葛欣琦 ，刘佳丽 ，舒汉鼎 ，陈婷婷 (1． 南昌大学 生命科学与食品工程学院，江西 南昌 330031 ;2 ． 南昌工程学院 水利与生态工程学院，江西 南昌 330099) 摘要:为认识黄颡鱼染色体组特征、建立种质标准和人工驯化养殖提供基础参数资料。以肾细胞制作染色体标 本，采用PHA 和秋水仙素活体注射，体内肾细胞培养法和空气干燥法对鄱阳湖黄颡鱼染色体核型进行分析。 结果表明:鄱阳湖黄颡鱼的2 倍染色体数为2n = 52 ，核型公式为2n = 20m + 14sm + 14st + 4t ，染色体总臂数 (NF)为86 。比较分析认为，黄颡鱼在鱼类进化史上属于低位特化类群。 关键词:黄颡鱼;染色体核型;特化类群 中图分类号:Q959 ． 483 文献标志码:A 文章编号:1000 － 2286 (2012)06 － 1222 － 04 Karyotype Analysis of Pelteobagrus f ulvidraco in Poyang Lake and Discussion of Its Evolutionary Position 1 1 2 1 1 Mao Hui-ling ，Ge Xin-qi ，Liu Jia-li ，Shu Han-ding ，Chen Ting-ting (1． College of Life Science and Food Engineering ，Nanchang University ，Nanchang 330031，China ;2． School of Hydraulic and Ecological Engineering ，Nanchang Institute of Technology ，Nanchang 330099 ，China) Abstract :The study aimed to provide basic data for understanding the chromosome characteristics of Pel- teobagrus f ulvidraco ，establishing its germplasm standard and its artificial domestication breeding． In order to make chromosome specimens with the kidney cells ，the karyotype of Pelteobagrus f ulvidraco in Poyang Lake was analyzed by using the methods of PHA and colchicine injection in vivo ，kidney cell culture and air drying． The results showed that Pelteobagrus f ulvidraco in Poyang Lake had a diploid number of 52 chromosomes． The karyotype formula was 2n = 20m + 14sm + 14st + 4t． NF = 86 ． Compared with the karyotypes of o