吖啶橙提高葡萄糖氧化酶生物电催化活性作用的研究.docVIP

吖啶橙提高葡萄糖氧化酶生物电催化活性作用的研究 摘 要 电化学生物传感器的发展是目前分析研究中最活跃的领域之一。由于其具有灵敏度高、选择性好、响应快、操作简便、样品需要量少、可微型化、价格低廉等特点而广泛的应用于医疗保健、食品工业、农业以及环境等领域。葡萄糖氧化酶（GOx）的特点决定了其成为生物传感器及生物燃料电池等生物电子器件中使用最广泛的酶之一。但葡萄糖氧化酶在水溶液中不太稳定，并且易受外界环境干扰而丧失活性。因此采用合适的固定技术将葡萄糖氧化酶固定在载体上成为生物电子器件研究的关键步骤，将直接影响酶电极的灵敏度、稳定性以及酶的活性与寿命等。本课题使用有机染料吖啶橙(AO)与葡萄糖氧化酶物理共吸附法将葡萄糖氧化酶固定于炭毡(CF)电极上（GOx/AO-CF），对吸附时间、葡萄糖氧化酶浓度、吖啶橙浓度、吸附液pH值、吸附液浓度、电解质溶液pH值等实验条件进行了优化与分析。与葡萄糖氧化酶单独吸附的炭毡电极(GOx-CF)比较，GOx/AO-CF电极展现出了优越的电化学性质。所制得的电极具有良好的操作稳定性，在使用葡萄糖进行连续10次测试中，电极的RSD%在4%以下。使用相同条件制备的三块炭毡电极，对葡萄糖进行测试，三块电极的RSD%为5%，因此我们认为应用此方法对炭毡电极进行固定具有很好的重现性。此外，将该电极储存在4度的环境中，一个月后该电极仍保持了初始活性的XXX。 cridine orange-induced enhancement effect of glucose oxidase for bioelectrocatalytic activity Abstract The development of electrochemical biosensor is one of the attractive fields in analysis research. It is widely applied in clinical, food industry, agriculture and environment because of its’ good characteristics such as high sensitivity, good selectivity, easy operation, small amount sample, miniaturization and cheap. Glucose oxidase (GOx) is the most popular enzyme which applied in biosensor and bio-fuel cell for its good character. But it is unstable in water and easy to denature because of the outer unfavorable condition. The proper immobilization method is the key procedure for GOx electrode which directly influencing the characteristics of electrode. We immobilized GOx on the carbon felt surface by the physical co-adsorption with acridine orange (AO). The adsorption time, GOx concentration, AO concentration, adsorption solution pH, adsorption solution concentration and electrolyte solution pH were optimized in this research. The GOx/AO-CF showed good performance as compared with GOx-CF. The RSD% of electrode is less than 4 after 10 times’ measurement by using D-glucose. The reproducibilities of three GOx/AO-CFs were prepared through the same conditions. The RSD% is 5 by using D-glucose. Then we can evaluate that it has good reproducibility by using this method. And the e