汉麻籽壳多酚的提取及抗氧化活性评价.pdfVIP

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.7 汉麻籽壳多酚的提取及抗氧化活性评价 1 1 1，2 2 李晓辉 ，刘海杰 ，陈天鹏 ，何锦风 （1.中国农业大学食品科学与营养工程学院，北京 100083） （2.总后勤部军需装备研究所军用汉麻材料研究中心，北京 100082） 摘要：汉麻籽壳是汉麻籽加工的副产物，其中含有丰富的抗氧化物质。本文以汉麻籽抗氧化多酚得率为指标，筛选出汉麻籽壳 抗氧化多酚提取的适宜条件，并对提取物的抗氧化性能进行评价。结果表明，汉麻籽壳中抗氧化成分最适宜提取的条件为液料比 （10:1 ）、提取时间（30 min ）、提取次数（3 次）、提取液浓度（60% 乙醇溶液）；市售大孔树脂中HPD-600 对汉麻籽壳抗氧化成分的 吸附能力最强；汉麻籽壳中抗氧化成分最适宜的洗脱条件为洗脱流速（1 mL/min ）的梯度洗脱，40% 乙醇洗脱溶液为目标产物；经过 大孔树脂分离提取的汉抗氧化多酚对于质粒 DNA、人血低密度脂蛋白（LDL ）和牛血清蛋白（BSA ）的氧化损伤都有显著的保护作 用。 关键词：汉麻籽壳；多酚；大孔树脂；抗氧化；氧化损伤； 文章篇号：1673-9078(2012)7-1007-1012 Extraction and Anti-oxidant Activity Evaluation of Polyphenol from Hempseed Hull 1 1 1,2 2 LI Xiao-hui , LIU Hai-jie , CHEN Tian-peng , HE Jin-feng (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) (2.The Research Center of China-Hemp Materials of the Quartermaster Research Institute of the General Logistic Department, Beijing 100082, China) Abstract: Hempseed hull, a by-product in hempseed oil processing, is rich in antioxidants. In this study, the conditions of polyphenol extraction from hempseed hull by ultrasonic were optimized on base of yield, and in vitro antioxidant properties of the extract were evaluated. Solvent extraction and macroporous resin absorption were employed in polyphenol separation. The results showed that the optimal extraction conditions were as follows: solid-liquid ratio 1:10, extraction time 30 min, extraction times 3 times and ethonal concentration 60%. HPD-600 was determin