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Chapter 1 Histology and Its Methods of StudyObjectivesGrasp firmly content and significance of histological research.Grasp generally effects of research methods of histology on development of the histology.Comprehend generally research method of histology; brief developmental history and developmental information of present age in histology; status of histology in medical curriculum.OverviewStudy-Focusing QuestionsMono-choice questions1-1 Which of the following techniques is most commonly used to locate glycogen in cells? CA.Methylene blue stainingB.Feulgen’s reactionC.Pariodic acid-Schiff (PAS) reactionD.Enzyme histochemistryE.Immunohistochemistry1-2 Techniques that permit direct observation of living cells include which of the following? CA.homogenization and differential centrifugationB.cryofracture and freeze etchC.phase contrast microscopy and tissue cultureD.radioautography and transmission electron microscopyE.in situ hybridization1-3 In situ hybridization is used to demonstrate the pattern of expression of which of the following types of molecule? DA.AntibodyB.CarbohydrateC.EnzymeD.Nucleic acidE.Protein1-4 Frozen sectioning may be required to avoid the removal of which of the following target substances when tissue are prepared for paraffin sectioning? DA.Basic proteinB.LipidsC.ZnymesD.CarbohydratesE.Nucleic acids1-5 Which of the following is enhanced by chemical fixation? BA.AutolysisB.Enzyme activityC.Structural preservationD.Immunohistochemical detection of tissue antigensE.Bacterial 1-6 Which of the following is the electron microscope component that corresponds with the condenser lens of the light microscope? CA.Fluorescent screenB.CathodeC.ElectromagnetD.AnodeE.Electron beam1-7 Of the following techniques, the one most commonly used to locate glycogen incells is: CA.Methylene blue stainingB.Feutgens reactionC.PAS reactionD.Enzyme histochemistryE.Immunohistochemistry1-8The most sensitive and specific method for localizing proteins in cells and t
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