afp 基因导入花粉管对杏果实发育影响的研究.docVIP

山东农业大学学报格式： 投稿体例：顺序依次为标题、作者、单位、摘要、关键词（3－5个），英文标题、作者、单位、摘要、关键词（3－5个），正文，参考文献 afp 基因导入花粉管对杏果实发育影响的研究 孙仲序1,冯殿齐2 ,刘　静2 ,赵进红2 ,王玉山2 ,张　玲1 ,马玉敏2 ,孙海伟2 (1. 山东农业大学园艺学院,山东泰安　271018 ;2. 泰安市泰山林业科学研究院,山东泰安 271000) 摘要:将afp 基因导入杏花粉管对果实发育产生的影响进行了研究,结果表明:处理后果实进入第一速长期多数较 对照推迟,最长7d ,最短3d ,提前3～11d 进入第二速长期。所有处理组合坐果率均较对照明显下降。果实含糖量 除组合1 与对照相等外,其余均较对照高,以组合7 最高,较对照高3. 8 %。各因素对坐果率和果实含糖量影响大 小依次为:切除花柱 授粉后间隔时间 DNA 浓度,影响坐果率以不切除花柱、DNA 浓度500μg/ ml 和授粉后间 隔1d 处理为最佳组合;影响果实含糖量以完全切除花柱、DNA 浓度2. 50μg/ ml 和授粉后间隔2d 处理为最佳组 合;影响果实发育以不切除花柱、DNA 浓度250μg/ mL 和授粉后间隔1d 处理为最佳组合。 关键词:杏;花粉管:afp 基因;果实发育 中图分类号:S335. 2 + 9 　　　文献标识码:A 　　　文章编号:1000 - 2324 (2005) 02 - 0161 – 06 THE STUDY OF EFFECT THE TRANSFORMED AFP GENEONDEVELOPMENTOF APRICOT FRUITS SUN Zhong - xu1 ,FEN G Dian - qi2 ,L IU J ing2 ,ZHAO J in - hong2 , WAN G Yu - shan2 ,ZHAN G Ling1 ,MA Yu - min2 ,SUN Hai - wei2 (1. College of Horticulture Shandong Agricultural University , Tai’an 271018 ,China ; 2. TaishanForest ry Science Institute , Tai’an 271000 ,China) Abstract :The alp gene was t ranstened into t he apricot pollen t ube ,it s effect on t he f ruit s development was st udried The result indicated t hat t he t reated f ruit s got into t he first rapid growing period later for 3 to 7 days t han t he colnparison , but got into the second rapid growing period earlier for 8 to 11 days t han t hat of t he comparison. Compared wit h t he comparison , f ruit bearing percentage of all t reatment s re2 markably declined , the saccharinity of the No. It reatment was equal to the comparison and t he ot her s’ all increased. The saccharinity of t he No. 7 is t he highest ,3. 8 %higher t han t hat of t he comparison. The or2 der f rom high to low of factor s effecting on fi?uit bearing rate and t he saccharinity is : cut the flower t ube interval af ter pollination DNA concent ration. The best group of the effect on bearing percentage is t he group wit h uncut pollen t ube , 500μ g / ml DNA concent ration and t reated af ter pollination for one day ; The