人卵泡液中干细胞因子水平及其对卵泡发育的影响.docVIP

人卵泡液中干细胞因子水平及其对卵泡发育的影响 作者：王桂红, 赵海波,辛晓燕 【关键词】 卵巢滤泡 　　【Abstract】 AIM： To investigate whether stem cell factor (SCF) exists in human follicular fluid (FF) and its effects on the development of ovarian follicles. METHODS： Enzyme-linked immunosobent assay (ELISA) was used to determine the levels of SCF in the serum and in FF obtained during oocyte pick-up (Opu) in 30 in vitro fertilization (IVF)-embryo transfer (ET) cases, which were divided into three groups: high response group, moderate response group and low response group. RESULTS: ① SCF levels in FF were significantly higher than those of matched serum levels during Opu ［(514±67) ng#12539;L-1, (395±49) ng#12539;L-1, respectively, P lt;0.05］; ② There were no significant changes of serum SCF level before and after superovulation (P gt;0.05). ③ While no significant difference of the SCF levels in FF was found in the high response group and moderate response group ［（564±64）ng#12539;L-1，（532±55）ng#12539;L-1，respectively］, significant difference was found between the above two groups and low response group ［（352±78）ng#12539;L-1， P lt;0.01］ and the levels were positively correlated with the number of developing follicles (r=0.412, P lt;0.05). CONCLUSION: SCF is produced by developing follicle itself and not permeated from the blood and it may play a complementary role in the regulation of follicle development. 　　【Keywords】 stem cell factor ; ovarian follicle; follicular fluid 　　【摘要】 目的： 探讨人卵泡液中是否存在干细胞因子（SCF）及其与卵泡发育的关系.方法： 采用夹心ELISA方法检测30例进行体外受精胚胎移植（IVFET）患者超促排卵前、取卵时血清与卵泡液中SCF的水平.结果: ① 取卵时卵泡液中SCF水平（514±67）ng#12539;L1，显著高于同期血清水平(395±49) ng#12539;L1，差别有显著性（P lt;0.05）;② 取卵时血清中SCF水平与超促排卵前血清中SCF水平相比，差别无显著性［（395±49）ng#12539;L1，（374±36）ng#12539;L1，P gt;0.05］; ③ 高、中反应型组卵泡中SCF水平［（564±64）ng#12539;L1，（532±55）ng#12539;L1］与低反应型（352±78）ng#12539;L1相比，差别有显著性（P lt;0.01），且与卵泡发育数目之间呈正相关（r=0.412，P lt;0.05）.结论: 卵泡液中SCF 可能来自于卵泡本身，而非血浆渗透.SCF参与人卵泡发育的调控. 　　【关键词】 干细胞因子;卵巢滤泡;卵泡液 　　0引言 　　卵泡的生长主要靠促性腺激素的刺激，近年来，