ELK1及其靶基因的影响.pdfVIP

·26· Chinese Journal of Information on TCM Jan.2008 Vol.15 No.1 榄香烯乳对人宫颈癌 Hela 细胞转录因子 ELK1 及其靶基因的影响 1 1 2 3 3 陈小军 ,顾立刚 ,李佩文 ,石太平 ,马大龙 (1.北京中医药大学免疫学教研室,北京 100029；2.中日友好医院中医肿瘤科,北京 100029； 3.国家人类基因组北方研究中心,北京 100176) 摘要：目的 观察中药榄香烯乳对人宫颈癌Hela细胞转录因子ELK1 及其靶基因的影响,以深入了解榄香烯乳的 抗癌机制。方法 用 MTT 法检测药物对细胞的抑制作用,双荧光素酶报告系统检测药物对转录因子 ELK1 的影 响,Western Blot检测磷酸化ELK1及其靶基因c-fos表达。结果 榄香烯乳能明显抑制人宫颈癌Hela细胞的生长, 其半数生长抑制剂量为80.6 µg/mL；榄香烯乳处理组荧光素酶活性下降,磷酸化的ELK1 及其靶基因c-fos的表达均 下调。结论 榄香烯乳可能通过下调转录因子 ELK1 的磷酸化水平,抑制 c-fos 的表达,从而抑制人宫颈癌 Hela 细胞 的生长。 关键词：榄香烯乳；Hela细胞；ELK1 中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2008)01-0026-02 1 1 2 CHEN Xiao-jun , GU Li-gang , LI Pei-wen , et al (1.Beijing University of TCM, Beijing 100029, China； 2.China-Japan Friendship Hospital, Beijing 100029, China) To study the effect of elemene on transcription factor ELK1 and its target gene in human cervix cancer Hela cell. The cell proliferation was assessed by MTT assay. The luciferase activity of transcription factor ELK1 was determined by the Dual-Luciferase Reporter Assay System. The protein expression of phosphorated ELK1 and its target gene c-fos were determined by Western Blot. Elemene can remarkably inhibited the growth of Hela cell and its IC50 was 80.6 µg/mL. The luciferase activity of transcription factor ELK1 in Hela cells treated with elemene was inhibited. The protein expression of phosphorated ELK1 and its target gene c-fos in Hela cells treated with elemene were down-regulated. Elemene can inhibit human cervix ca