临床研究不同分子分型乳腺癌干细胞MDR1表达的研究.pdfVIP

·1636 · 南方医科大学学报（J South Med Univ ） 2012;32(11) 临床研究 不同分子分型乳腺癌干细胞MDR 1表达的研究 杨国华，薛芳沁，陈晓耕 福建医科大学省立临床学院肿瘤外科，福建 福州 350001 摘要：目的探讨乳腺癌干细胞MDR 1表达与乳腺癌不同分子分型的关系。方法 根据ER、PR、Her-2 及CK5/ 14 的水平划分为5 个分子亚型，分为5组，通过PCR 法分析不同分子分型乳腺癌组织中癌干细胞MDR 1表达，分析乳癌干细胞中MDR 1表达量与 乳腺癌不同分子分型的关系。结果 Luminal A 型组癌干细胞MDR 1量（0.26±0.04）和Luminal B 型组（0.31±0.03）最少，两组 MDR 1量无显著性差异（P0.05）；HER-2+ 型组乳腺癌干细胞MDR 1量（0.56±0.05）明显多于A 组和B 组，具有显著性差异（P 0.05）；Basal-like 型癌干细胞的MDR 1量（0.98± 0.01）和Normal-like 型的MDR 1量（0.90± 0.15）最多，两组无显著性差异（P 0.05），但明显多于Luminal A 型/B 组、HER-2（+ ）型组，具有显著性差异（P0.05）。结论 乳腺癌干细胞MDR 1表达与其分子分 型有关。 关键词：乳腺癌；癌干细胞；多药耐药；分子分型 中图分类号：R737.9 文献标志码：A 文章编号：1673-4254（2012）11-1636-03 doi: 10.3969/j.issn. 1673-4254.2012.11.025 Expression of MDR1 gene in cancer stem cells in breast cancer tissues of different molecular subtypes YANG Guohua, XUE Fangqin, CHEN Xiaogeng Department of Cancer Surgery, Provincial Clinical College, Fujian Medical University, Fuzhou 350001, China Abstract: Objective To investigate the association between MDR1 gene expression in breast cancer stem cells and the molecular subtypes of breast cancer tissue. Methods According to ER, PR, Her-2 and CK5/14 expression profiles, 153 breast cancer specimens were divided into 5 molecular molecular subtypes, in which the expression of MDR1 was detected to analyze the relationship between MDR1 gene expression and the subtypes of breast cancer stem cells. Results The expression of MDR1 in Luminal A subtype breast cancer was 0.26 ± 0.04, which showed no significant difference from that of Luminal B subtype (0.31±0.03, P0.05). Compared with these two subtypes, HER-2 (+) subtype breast cancer tissues showed a significantly higher MDR1 expression(0.56 ± 0.05, P0.05). MDR1 expression in Basal-like subtype and Normal-like subtype br