固定化细胞技术及其应用研究进展.pdfVIP

· 576 · 生命的化学, 2013, 33(5): 576-580 《生命的化学》2013 年33 卷5 期 综述 固定化细胞技术及其应用研究进展 1# 2# 1* 奚悦 ，焦姮 ，刘小宇 1 2 ( 第二军医大学基础部生化教研室，上海200433；复旦大学上海医学院，上海200433) 摘要：细胞固定技术是将具有特定生理功能的生物细胞用一定的方法进行固定，并以其作为生物催化 剂加以利用的一门技术。相对于游离的单细胞，固定化细胞可简化生产工艺，降低生产成本。本文回 顾了细胞固定技术在制备方法和载体材料等方面的研究进展，并总结了近几年来固定化细胞技术在新 能源开发、食品加工及环境污染物处理中的应用，对其发展前景进行展望。 关键词：固定化细胞技术；制备方法；固定化载体；应用 Cell immobilization technique and its application development XI Yue1#, JIAO Heng2#, LIU Xiaoyu1* 1 ( Department of Biotechnology and Molecular Biology, Department of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China; 2 Shanghai Medical College of Fudan University, Shanghai 200433, China) Abstract: The immobilized whole cell system is a biocatalyst system where the target cell is attached to a solid support (such as calcium alginate). Compared with ordinary biocatalysts systems, immobilized whole cell systems simplify the industrial production process and reduce production costs. In this paper, the methods and carriers for cell immobilization technique are introduced, and its applications in the development of new energy, industrial production and waste treatment in these years are summarized. And we also discuss the prospect of the application of this technique. Key words: cell immobilization technique; preparation method; carrier; application 固定化细胞技术，是利用物理或化学手段将 化枯草芽孢杆菌(Bacillus subtilis)成功生产了淀粉 ( 具有一定生理功能的生物细胞 微生物细胞、植物 酶。目前，细胞固定化技术的应用范围已涵盖生 ) 细胞或动物细胞等