濒危植物珙桐的组织培育技术研究.pdfVIP

安徽农业科学,Journal of Anhui Agri . Sci .2007 ,35( 18) :5369 ,5372 责任编辑 罗芸 责任校对 李菲菲 濒危植物珙桐的组织培养技术研究 * 李月琴, 雷泞菲, 林莎, 秦小波, 徐莺 , 陈放 ( 四川大学生命科学学院, 四川成都610065) 摘要 以珙桐成 熟胚为试材, 研 究了不 同生长调节剂组合对珙桐成 熟胚 离体分化的影响, 并获得 了完整的植株 。结果表 明: 诱导愈伤组 织的最佳培养基组合为 1/ 2MS + 0 .5 mg/ L 6- BA + 3 mg/ L IBA + 1 mg/ L GA + 蔗糖30 g/ L ; 诱导不定 芽的最佳培养基组合为 1/ 2MS + 1.0 3 mg/ L6- BA + 蔗糖20 g/ L ; 最有利于不定芽的增殖与伸长的培养基组合为 MS + 0 .5 mg/ L 6- BA + 1.0 mg/ L GA + 蔗糖20 g/ L ; 诱导生根 的最 3 佳培养基组合为 1/ 2 MS + 0 .8 mg/ L IBA + 0 .2 mg/ L NAA + 蔗糖10 g/ L 。 关键词 珙桐; 成熟胚; 组织培养 中图分类号 Q943 .1 文献标识码 A 文章编号 0517 - 66 11( 2007) 18 - 05369 - 0 1 Study on the Rare and Endangered Plant Davi di a i nvol ucr at e Baill Tissule Culture LI Yue- qin et al ( College of Life Sciences ,Sichuan University ,Chengdu ,Sichuan 6 10065) Abstract The effect of hor mone on the in vitro differentiation of mature embryos of Davi di a involucrat a Baill was studied .And an efficiency regeneration system of mature embryo was established .The results showed the opti mum mediumfor callus induction from mature embryo was 1/ 2MS + 0 .5 mg/ L6- BA + 3 mg/ L IBA + 1 mg/ L GA + sucrose 30 g/ L .The opti mum mediumfor adventitious bud was 1/ 2MS + 1 .0mg/ L 6- BA + sucrose 20 g/ L.The opti mum 3 mediumfor shoot propagation and elongation was MS + 0 . 5 mg/ L 6- BA + 1.0 mg/ L GA + sucrose 20 g/ L ;and the adventitious root was induced on 3 1/ 2MS + 0 .8 mg/ L IBA + 0 .2 mg/ L NAA + sucrose10 g/ L. Key words Davi di a involucr ate Baill ;Mature e mbryo ;Tissue cult