黄芩苷提取精制工艺研究.pdfVIP

第32卷第2期　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 中南民族大学学报(自然科学版)　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 Vol.32 No.2 2013年6月　 　 　 　 　 　 　 　 　 　 　 Journal of South-Central University for Nationalities(Nat.Sci.Edition)　 　 　 　 　 　 　 　 　 　 Jun.2013 黄芩苷提取精制工艺研究 廖矛川,罗会畏,李　 竣,高瑞希,邹大江,黄先菊 (中南民族大学药学院,武汉430074) 摘　 要　 为明确黄芩中黄芩苷提取和精制的最佳工艺,以水、60%乙醇、碱水为溶剂对黄芩进行了回流提取,浸膏 分别采用酸沉工艺和D101吸附纯化工艺精制,用高效液相色谱法测定黄芩出膏率,黄芩苷的得率和含量. 结果表 明:大孔树脂精制醇提物黄芩出膏率最高,为20.73%;酸沉淀法精制醇提物黄芩苷得率最高,为11.93%;酸沉淀法 精制水提取物黄芩苷含量最高,为69.29%.说明以大孔树脂精制醇提物和酸沉法精制水提物,能获得较高的黄芩 出膏率和黄芩苷含量. 关键词　 黄芩;黄芩苷;高效液相色谱法;提取工艺;纯化工艺 中图分类号　 TQ460.6;R284.1　 文献标识码　 A　 文章编号　 1672-4321(2013)02-0069-04 Study on the Extraction and Refining Technology of Baicalin from Scutellaria baicalensis Georg Liao Maochuan,Luo Huiwei,LiJun,Gao Ruixi,ZhouDajiang,Huang Xianju (College of Pharmacy,South-Central University for Nationalities,Wuhan430074) Abstract　 To investigate the best extraction and refining technology of baicalin from Scutellaria baicalensis Georg, Scutellaria baicalensis Georg wasreflux extractedwithwater,60% ethanol and alkalinewater,thenrefined andpurifiedby acid precipitation and D101 macroporous resin. HPLC was used to determine the extraction rate,yield and content of baicalin. The results indicated that extraction rate of Scutellaria baicalensis Georg in macroporous resin refined alcohol extract was the highest,about20.73%;baicalin yield in acid precipitation refined alcohol extract was the highest,about 11.93%; baicalin content in acid precipitation refined water extraction was the highest, about 69. 29%. With macroporous resin refining alcohol extract and acid sinking method purifying water extract of Scutellaria baicalensis Georg, we can obtain higher extract yield and content of ba