白屈菜碱诱导人胃癌SGC-7901细胞G2_M期阻滞及有丝分裂灾难死亡作用研究.docVIP

  白屈菜碱诱导人胃癌 SGC-7901 细胞 G2/M 期阻滞及有丝分裂灾难死亡作用研究# 曲中原1，邹翔2，季宇彬3** 5 （1. 哈尔滨商业大学药学院，哈尔滨 150076； 2. 哈尔滨商业大学药物研究所博士后科研工作站，哈尔滨 150076； 3. 哈尔滨商业大学抗肿瘤天然药物教育部工程研究中心，哈尔滨 150076） 摘要：目的: 研究白屈菜碱诱导人胃癌 SGC-7901 细胞的 G2/M 期阻滞及有丝分裂灾难死亡 10 15 作用。方法：采用 MTT 法检测白屈菜碱对 SGC-7901 细胞的增殖抑制作用；流式细胞仪检 测白屈菜碱对 SGC-7901 细胞的周期阻滞作用；采用 Giemsa 染色观察细胞核的变化。结果： 白屈菜碱能够显著抑制 SGC-7901 细胞的增殖，IC50 为 48.67μmol/L；白屈菜碱（25μmol/L） 作用 24h 后，G2/M 期细胞比例显著升高，与对照组比较差异有统计学意义(P0.01)。6.25～ 25μmol/L 白屈菜碱作用后，随着给药浓度的增加，多核大细胞明显增多。结论：白屈菜碱 可诱导 SGC-7901 细胞发生 G2/M 期阻滞，细胞出现多核大细胞增多等有丝分裂灾难死亡的 基本特征。 关键词：中药学；白屈菜碱；人胃癌 SGC-7901 细胞；G2/M 期阻滞；有丝分裂灾难 中图分类号：R282 20 G2/M pahse arrest and Mitotic catastrophe induced by chelidonine on human gastric carcinoma SGC-7901 cells QU Zhongyuan1, ZOU Xiang2, Ji Yubin3 (1. Pharmaceutical School, Harbin University of Commerce, Harbin 150076; 25 30 35 40 2. Postdoctoral Programme of Pharmacology Institute, Harbin University of Commerce, Harbin 150076; 3. Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076) Abstract: Objective: To study the G2/M phase arrest and mitotic catastrophe induction effect of chelidonine on human gastric carcinoma SGC-7901 cells. Methods: MTT assay was applied to detect the proliferation inhibition effect of chelidonine. Flow cytometry was applied to detect the cell cycle changes of SGC-7901 cells. Giemsa staining was used to observe the morphological changes of the nucleus in gastric cancer cells. Results: The results showed that chelidonine could effectively inhibit the proliferation of SGC-7901 cells and the IC50 was 48.67μmol/L. After treatment with 25μmol/L of chelidonine for 24 h, the ratio of G2/M phase cells was increased significantly which was statistically significant difference compared with that of the control group (P0.01). After treatment with 6.25-25μmol/L chelidonine, the numbers of giant cells with two or more nuclei was increased along with the increase of drug concentration. Conclusion: The conclusion can