青枯雷尔氏菌无致病力菌株诱导烟草植株防御酶系的变化.docVIP

不用方程，用误差线。 要分析比值的意义，做1的等值线，分析大于或小于1的意义。 讨论要理清思路写。 青枯雷尔氏菌胁迫对烟草植株抗氧化酶系活性的影响( 车建美1,2，刘波1*，张彦1,3，蓝江林1苏明星1，林抗美1 （1. 福建省农业科学院农业生物资源研究所，福州，350003；2. 福建农林大学生物农药与化学生物学教育部重点实验室，福州，350002；3. 福建农林大学植物保护学院，福州，350002） 摘 要：青枯雷尔氏菌RS-1403作为外源微生物胁迫接种后，采用不同浓度胁迫接种后的烟草植株叶片PPO和POD酶活性总和显著高于对照（P≤0.05），而SOD酶活性总和除了处理2（接种浓度为2.35×104CFU/mL）外，其余的各个处理的均低于对照。烟草植株的根部、茎部和叶片的POD、PPO和SOD酶活性总和明显高于对照处理，并且其酶活性达到高峰的时间有所不同。胁迫接种5 d后，烟草植株叶片和茎部的POD酶活性和对照处理的比值最大，胁迫接种10 d后，烟草植株根部POD酶活性比值最大。烟草植株叶片和根部在接种15 d后，PPO酶活性和对照处理的比值达到最大，茎部PPO酶活性在接种5 d后比值最大。接种20 d后，处理烟草植株叶片和茎部的SOD酶活性和对照处理的比值达到最大，接种后25 d，根部SOD酶活性比值最大。 关键词：青枯雷尔氏菌，无致病力菌株，烟草，过氧化物酶（POD）、多酚氧化酶（PPO）、超氧化物歧化酶（SOD） Effects of avirulent Ralstonia solanacearum strain on some antioxidant enzymes activities in tobacco plants Che Jianmei1,2, Liu Bo1*, Zhang Yan1,3, Lan Jianglin1, Su Mingxing1, Lin Kangmei1 （1. Biotechnology Institution, Fujian Academy of Agricultural Sciences, Fuzhou 350003,China; 2. Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002; 3.The plant protection college, Fujian Agriculture and Forestry University, Fuzhou 350002） Abstract: The effects of avirulent Ralstonia solanacearum strain RS-1403 on the activities of guaiacol peroxidase (POD; EC )，polyphenol oxidase (PPO; EC ) and superoxide dismutase (SOD; EC ) were investigated in tobacco plants. Higher levels of total POD and PPO activities were observed in tobacco leaves treated with different concentrations of avirulent R. solanacearum strain RS-1403 compared with control (P≤0.05). Total SOD activity of tobacco leaves with different concentrations of avirulent R. solanacearum strain RS-1403 was lower than control except that with the concentration of 2.35×104CFU/mL. The total POD, PPO and SOD activities of tobacco plants different parts including the roots, leaves and stems were significantly higher than that of control. The times of reaching peak of POD, PPO and SOD activity ratios with that of control in different parts of tobacco plant