斜带石斑鱼囊胚期胚胎和尾芽期胚胎 差异表达基因的筛选及克.pdfVIP

2006, Oct. 27 (5) : 461- 472 CN 53- 1040/ Q ISSN 0254- 5853 Zoological R s arch 1, 2 1 1 1, * 汲广东 , 李名友, 周 莉, 桂建芳 ( 1. , 430072; 2. , 100049) : , 8 7 cDNA A-tubulin , 2 2 192 960 PCR , 15 131 , 15 11 cDNA 4 cDNA ; 131 , 123 cDNA 8 cD- NA RT-PCR ; ; ; ; : Q9591483; Q785 A 0254- 5853 ( 2006) 05- 0461- 012 : : : Scr ning and Analysis of Diff r ntial Expr ssion G n s in Blastula and Tail Bud Stag Embryos in th Orang- spott d Group r ( Ep inep helus coioides ) 1, 2 1 1 1, * J I Guang-dong , LI Ming-you , Z HOU Li , GUI Jia n-fang (1. S tate Key Laboratory of Freshwater Ecology and Biotechnology , Wuhan Centerf or Develo mental Biology , Institute of Hydrobiology, Chinese A cademy of Sciences , Wuhan 430072, China; 2 . Graduate S chool of the Chinese A cademy of Sciences, Beij ing 100049, China) Abstract : Suppre ion ubtractive hybridization ( SSH ) librarie were con tructed in bla tula tage and tail bud tage embryo in the orang e- potted grouper ( E ine helus coioides) . T he ubtraction efficiency of the two ubtractive cDNA l-i 8 7 brarie indicated by A-tubulin cDN