毒品成瘾与脑组织基因表达谱的研究进展.pdfVIP

HEREDITAS (Beijing) 2008 7 , 30(7): 809―814 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2008.00809 陈峰, 李涛, 樊栓良, 党永辉, 陈腾, 阎春霞 , , , 710061 : 毒品成瘾是由滥用毒品外在因素与遗传易感性等内在因素共同作用而导致的一种慢性脑疾病; 毒品成瘾 的机制目前还不十分清楚。毒品成瘾研究的一个主要目标是鉴别和分离毒品导致脑功能障碍的分子机制; 采用 高通量的基因表达谱技术研究毒品成瘾者在不同状态下脑基因表达全貌, 对深入认识毒品成瘾的机制具有重 要的意义。文章综述了毒品成瘾的遗传机制及高通量脑组织基因组表达技术——SAGE 和微阵列(Microarry)在 毒品成瘾研究中的进展。 : 毒品滥用; 毒品成瘾; 基因表达谱; 基因表达系列分析(SAGE); 微阵列; 生物信息学 Gene expression profiling in drug addicted brain CHEN Feng, LI Tao, FAN Shuan-Liang, DANG Yong-Hui, CHEN Teng, YAN Chun-Xia Department of Forensic Sciences ; Key Laboratory of the Health Ministry for Forensic Sciences; Key Laboratory of Environment and Genes Related to Diseases of the Education Ministry, Xi ’an Jiaotong University School of Medicine, Xi ’an 710061, China Abstract: Drug addiction, a chronic brain disease caused by interaction of in vitro drug toxification and in vivo gene sus- ceptibility, has been widely studied but its underlining mechanism is so far been elucidated. A major goal in this field is to identify drug-induced molecular changes and their effects on brain function. By the advance of high throughput technolo- gies in genomics and transcriptomics, the whole gene expression profile in addicted brain could be obtained and proved to be a very powerful tool to unclose the molecular mechanism underlying the addiction biology context. Here, we summarized the progress of serial analysis of gene expression (SAGE) and microarray, as well as their application in drug addiction. Keywords: drug abuse; drug addiction; gene expression profiling; serial analysis of gene expression (SAGE); microarray; bioinformatics