荧光光谱法研究木栓酮与牛血清白蛋白的相互作用.pdfVIP

2012-04-25 DOI: 10.3724/SP.J.1145.2012.00287 / Chin J Appl Environ Biol 2012 18 ( 2 ) : 287~291 * 1 2 1, 2 2 1** FriedelinFDLBovine serum albuminBSA. . 1 : 1. . Förster 2.86 nm. . 5 2 12 CLC R963 : Q946.41 SSpectroscopic Studies on Interaction of Friedelin withpectroscopic Studies on Interaction of Friedelin with BBovine Serum Albuminovine Serum Albumin* 1 2 1, 2 2 1** LIN Zhiwei , TAI Zhengfu , WAN Zhongmin , WANG Fei LEI Ningfei 1 ( College of Materials and Chemistry and Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China) (2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) AAbstractbstract In order to find the transport mechanism of triterpenoids in vivo, the fluorescence and UV-Vis spectroscopic methods were used to study the binding reaction between friedelin, a pentacyclic triterpenoid isolated from Rostellularia procumbens , and bovine serum albumin (BSA). The fluorescent emission of BSA was inhibited by friedelin in a dose- dependent manner, indicating that friedelin interacted with BSA by a static process. The binding constant and the number of binding sites were calculated based on the quenching effect of freidelin on BSA, and the results indicated that friedelin interacted with BSA in high affi nity with the binding ratio of 1 : 1 under alkalescent condition. Thermodynamic parameter study indicated the interaction force between friedelin and BSA was hydrogen bo