长春花资源遗传多样性的ISSR分析Thegeneticdiversityof.pdfVIP

6th OAPS Working Paper Series Paper No. 2009-033 Website: http://www.oaps.hk/ 长春花资源遗传多样性的ISSR 分析 The genetic diversity of Catharanthus roseus germplasm revealed by ISSR markers 潘彬也 Pan Binye 农业与生物学院 School of Agriculture and Biology 摘要 长春花 (Catharanthus roseus) 为夹竹桃科（Apocynaceae）长春花属(Catharanthus) 植物， 常绿草本，花色鲜艳。本文应用ISSR 标记研究长春花资源的遗传多样性。选取40 个长春花材料， 用筛选出的20 个ISSR 引物进行PCR 扩增，共扩增出115 条重复性高、清晰的条带，扩增片断分子 量从200 bp 至 1200 bp，平均每个引物扩增出5.75 条带，其中多态性条带为78 条，有效条带百 分率(P)67.8％。基因多样性指数(H)为0.3735，Shannon 多样性指数(I)为0.5546。在居群水平上， 基因多样性指数(H)为 0.0718-0.4999，Shannon 多样性指数(I)为 0.1592-0.6931，说明所选的长 春花品种间具有较高遗传多样性。在居群水平上，40个长春花品种Jaccard遗传相似性系数在0.150 到0.900 之间，平均为0.584。居群间的遗传距离UPGMA 结果显示，以0.588 为阈值可以把40 个 长春花材料分为七组，各公司最初育种材料多群聚成一组中。实验结果还表明，用 ISSR 进行 PCR 扩增，可以清晰、有效地分辨出每个样品的基因型，说明 ISSR 标记适用于长春花的遗传多样性研 究。此研究结果将为品种的选育和推广利用提供一定的依据。 关键词：长春花，ISSR，遗传多样性 ABSTRACT Catharanthus roseus (Madagascar Periwinkle) is a species of Catharanthus native and endemic to Madagascar. It is an evergreen subshrub or herbaceous plant, with colorful flowers. In the present study, we used inter-simple sequence repeats (ISSR) to examine the genetic diversity of Catharanthus roseus germplasm. We sampled 40 individuals. Twenty ISSR primers were selected for PCR and produced 115 band loci, of which 78(P=67.8%)were polymorphic. The average number of bands per prime was 5.75 and the size of the amplified DNA fragments ranged from 200 bp to 1200 bp, showing high level of genetic differentiation. At species level, the coefficient of Jaccard genetic similarity ranged from 0.150 to 0.900,with the average of 0.584. 40 individuals we