菌株hp3 对溴胺酸的降解原理.pdfVIP

中国环境科学 2001,21(2) 180 184 China Environmental Science 菌株HP3 对溴胺酸的降解机理 黄丽萍,周集体,王 竞,杨凤林 大连理工大学环境科学与工程学院,辽宁 大连 116012 摘要 通过正交试验,得到菌株HP3 降解溴胺酸最佳条件为温度30 ,pH 值7.0,摇床转速 160r/min ,培养基中不含NaCl 有利于溴胺酸的降 解.在此条件下,溴胺酸的降解遵从负指数模式.葡萄糖和硫酸铵的加入显著地提高菌体降解溴胺酸的速度.不同金属化合物对菌株 HP3 降 解溴胺酸有不同程度的抑制作用,其中以HgCl2 和AgNO3 最显著.菌株HP3 能降解化合物蒽醌 1,4,5,8-四羟基蒽醌和1-氨基蒽醌-2-磺酸钠, 表明该菌株对底物的降解没有严格的专一性.溴胺酸经菌株HP3 作用后吸收光谱发生明显变化.TOC 分析表明,菌株HP3 将溴胺酸的蒽醌 环破坏后利用其中的一部分有机碳.液相色谱检测有中间产物邻苯二甲酸生成.溴胺酸降解终产物分子量为289 和290. 关键词 溴胺酸 降解速度 菌生物量 降解机理 邻苯二甲酸 中图分类号 X703 文献标识码 A 文章编号2001 Degradation and its mechanism of 1-amino-4-bromoanthraquinone-2-sulfonic sodium by strain HP3. HUANG Li-ping, ZHOU Ji-ti, WANG Jing, YANG Feng-lin (School of Environmental Science and Engineering, Dalian Univer- sity of Technology , Dalian 116012, China). China Environmental Science. 2001,21(2) 180 184 Abstract The optimal degradation conditions of 1-amino-4-bromoanthraquinone-2-sulfonic sodium(ABAS) by strain HP3 temperature=30 , pH=7.0, speed of shake-bed=160r/min and NaCl=0 in the culture basis were obtained by or- thogonal test. Under these conditions, the ABAS degradation followed negative exponential model. The addition of glu- cose and (NH ) SO could greatly enhance the degradation rate of ABAS by the strain. All experimental metal compounds 4 2 4 restrained the degradation of ABAS in different degrees, in which HgCl and AgNO could control the degradation most 2 3 obviously. Strain HP3 also degraded anthraquinone, 1,4,5,8-tetrahydroanthraquinone and 1-animoanthraquinone 2-sulfonic sodium. Degraded by HP3, the absorption spectrum of ABAS changed greatly showing that the degradation was not concentrated on single basis. TOC analysis showed a part of organi