苦参对鸡大肠杆菌的抑菌作用及其原理研究.pdfVIP

中国农业科学 2006,39(5):1018-1024 Scientia Agricultura Sinica 苦参对鸡大肠杆菌的抑菌作用及其机理研究 王关林，唐金花，蒋 丹，方宏筠，刘照斌 (辽宁师范大学生命科学学院，大连 116029) 摘要：【目的】阐明苦参的抑菌作用及机理，为生产实践提供理论依据。【方法】采用牛津杯法及液体倍比稀 释法对鸡大肠杆菌等常见致病细菌进行了敏感性试验；通过电镜观察，SDS-聚丙烯酰胺凝胶电泳对蛋白表达的研 究，生长曲线的绘制以及流式细胞仪对菌体细胞周期的分析，对苦参碱粗提物的抑菌作用及机理进行研究。【结果】 苦参对革兰氏阳性菌、革兰氏阴性菌均有明显抑制作用，抑菌谱广；药物是通过抑制鸡大肠杆菌功能蛋白的表达， 从而影响菌体的细胞周期，使 I 期菌数增加，R 期菌数下降，最终菌体不能达到正常对数生长期而直接进入衰亡 期；菌体形态结构发生明显变化，表现为菌体缢缩变形，中间凹陷呈规则元宝状，细胞质固缩，质壁分离，最终 细胞壁破损，内容物泄漏而使菌体死亡。【结论】苦参碱对鸡大肠杆菌抑菌机理是抑制调控细菌生长、分裂相关蛋 白的合成，阻滞其分裂和生长，并且与细胞内的蛋白质结合，细胞质固缩、解体而死亡。 关键词：苦参；苦参碱；抑菌作用；抑菌机理 Bacteriostatic Action and Mechanism of Sophora flavescens Ait on Escherichia coli 01 C84010 WANG Guan-lin, TANG Jin-hua, JIANG Dan, FANG Hong-jun, LIU Zhao-bin (Liaoning Normal University, Dalian 116029) Abstract: 【Objective 】An experiment was carried out to study the bacteriostatic action and mechanism of S.flavescens on E.coli. 01c84010. 【Method 】Cylinder-plate and liquid doubling dilution, drafted curves, electron microscope, SDSand flow cytometry, were ued in the study. 【Results 】The result showed that the extracts had obvious bacteriostatic action upon Gram-positive and Gram-negative bacteria and the bacteristatic spectrum was wide. Though restraining the expression of functional protein, the cell cycle was effected and the count of bacteria in phase I increased and that in phase R reduced. Finally, the E.coli 01 C84010 went into decline phase straightly instead of the normal logarithmic phase. Observation under electron microscope revealed that the E.coli 01 C84010, which depressed on center had been constricted into shoe-shaped gold ingots. The cytoplasm was concentrated, the cell was plasmolysed, then the cell wall was damaged, the contents were leaked out and the cell was