HGF和VEGFR-3在大肠癌中的表达及其在淋巴管生成和转移中的作用.pdfVIP

· 838 · 肿瘤防治研究2()09年第36卷第 1()期 DOI：10．3971／j．issn2009．…．()( 临床研究 HGF和 VEGFR一3在大肠癌 中的表达及其在 淋 巴管生成和转移 中的作用 熊枝繁 ，于 欢，曹仕琼，曾 劲，熊 亮 ExpressionofHGFandVEGFR-3inColorectalCarcinomaandTheirRoleinLymphangio— genesisandLymphaticM etastasis XIONGZhi—fan，YU Huan，CA()Shi—qiong，ZENGJin，XIONG Liang DepartmentofDigestion，LiyuanHospital，TongjiMedicalCollege，HuazhongUniversityofScience andTechnology．Wuhan430{}77。( ina Abstract：Objective Toinvestigatetheroleofhepatocytegrowthfactor(HGF)andvascularendothelial growthfactorreceptor3 (VEGFR～3)in]ymphangiogenesisandlymphaticmetastasisofcolorectaIcarci— nomabyanalyztheexpressionofHGFandVEGFR一3，andcalculatinglmy phaticvesseldensity(LMVD) ineolorectaIcarcinoma．Methods TheexpressionofHGF andVEGFR 3in specimensof52coIorectal carcinoma，20co／orectalpolypstissuesand20colorectalnormaltissueswasevaluatedbyimmunohisto— chemistryutilizingstreptavid~n-biotincomplex．LMVD incolorectalcarcinomawascalculatedbyusing podoplaninasthespecificmarkeroflymphaticendothelium．Andtheclinic。pathol。gica1featuresofcolor— ectalcarcinomawereanalyzed．Results (1)Thestainingsofbrownandfilemotincytoplastwereobserved astheposit~veexpressionofHGFandVEGFR一3proteins．Thepositiverate(75少 ，67．5 )andnleanval— Her(()．36±(J。07)，()《．41±()．1())]。fHGFandVEGFR一3expressionsinspecimensofco]orecta1carcino— maweresignificantlyhigherthanthoseofthecolorectalpolypstissues[25 ．3() ；(()．24±0．06)，(()．28 ±0．03)]andthenormalboweltissues(15 1() ；()．23±0．060．21±0．03)(P ()．05)．FheLMVDof colorectalcarcinomawasalsosignificantlyhigherthanthoseofthecolorectalpolypstissuesandthenor— ma1bowe1tissuesE(4．13±1．99)vos．(2．59±1．46)vs．(2．40±1．44)，P()．o11．(2)Asignificantcot- relationwasfoundbetweeneithertwoofLM VD，themeanvalueofHGFandVEGFR 3inspecimensof colorectalcarcinoma．ThepositiveexpressionofHGFwasfoundin39casesofcolorectalcarcinoma，and theirmeanLM