厚朴与远志配伍对胃肠组织NO和胃窦血管活性肠肽的影响.pdfVIP

392 食品与药品 Food and Drug 2011年第 13卷第 11期 厚朴与远志配伍对胃肠组织NO和胃窦血管活性肠肽的影响 * 傅 勇，肖 武，王 建 ，刘莉娜，王丹丹，王 茜 （成都中医药大学药学院中药教研室，四川 成都 610075 ） 摘 要：目的 考察远志与厚朴配伍对胃肠组织一氧化氮 （NO ）、胃窦血管活性肠肽 （VIP ）的影响，探讨厚朴缓解远志 胃排空抑制的作用机制。方法 采用硝酸还原酶法，考察了远志、厚朴、远志与厚朴1:1、1:2 、1:3组对大鼠胃肠组织NO含 量的影响；采用免疫组织化学法，考察了给药组对大鼠胃窦肌层VIP 的影响。结果 远志能显著增加大鼠胃窦组织NO含 量；与单味远志组比较，远志与厚朴配伍各组均能显著降低的胃窦组织NO含量，但对十二指肠组织NO含量无显著影响。 单味远志能明显增强大鼠胃窦肌层的VIP免疫阳性产物表达；远志与厚朴配伍1:1组的VIP表达量明显低于单味远志。结论 单味生远志导致胃排空障碍可能与其增强胃窦组织NO含量、增强该区域肌层VIP高表达有关；厚朴可能通过降低胃窦NO 含量、下调胃窦区VIP表达发挥缓解其胃排空抑制作用的。 关键词：远志；厚朴；配伍；一氧化氮；血管活性肠肽 中图分类号：R285 文献标识码：A 文章编号：1672-979X （2011）11-0392-04 Effects of Compatibility of Magnolia Officinalis Cortex and Polygalae Radix on NO Content of Gastrointestinal Tract Tissues and VIP Content of Sinus Ventriculi FU Yong, XIAO Wu, WANG Jian, LIU Li-na, WANG Dan-dan, WANG Qian (Teaching and Research Section of Traditional Chinese Medicine, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China) Abstract: Objective To investigate the effects of the compatibility of Magnolia Officinalis Cortex and Polygalae Radix on nitrogen monoxidum (NO) of gastrointestinal tract tissues and vasoactive intestinal peptide (VIP) of stomach, and explore the mechanisms of Magnolia Officinalis Cortex on relieving the inhibitory action of gastric emptying that induced by Polygalae Radix. Methods The effects of Polygalae Radix, Magnolia Officinalis Cortex, and the compatibility of the two herbs (with the ratios of 1:1, 1:2 and 1:3) on NO content of gastrointestinal tract tissues of rats were investigated by using nitrate reductase method. The effects of the above mentioned herbs on VIP content of muscular layer of sinus ventriculi of rats