急性胰腺炎时腺泡细胞凋亡与Bcl-2 表达.docVIP

精品文档 值得下载急性胰腺炎时腺泡细胞凋亡与Bcl-2表达﹡谢荣俊① 张树友① 李峰① 周筱筠① 刘龙飞① 卢先州①①南华大学附属南华医院 湖南 衡阳 421001谢荣俊﹡基金项目:湖南省卫生厅资助项目(B2007115)【摘要】 目的：探讨急性胰腺炎(AP)时腺泡细胞凋亡与Bcl-2表达的关系。方法：SD大鼠30只,随机分为假手术组(A组)、急性水肿性胰腺炎组(B组)、急性出血坏死性胰腺炎组(C组)。采用逆行胰胆管穿刺注射法建立急性水肿性与出血坏死性胰腺炎模型。检测造模后12 h各组的血清淀粉酶活性，腹水情况，胰腺组织大体、镜下病理改变与评分，腺泡细胞凋亡指数(AI)，Bcl-2蛋白表达情况。结果：⑴C组血清淀粉酶活性、腹水量、大体及镜下病理评分、Bcl-2阳性率显著高于B组(P＜0.05)，腺泡细胞AI显著低于B组(P＜0.05)。⑵B、C组中腺泡细胞AI与大体及镜下病理评分均呈显著负相关关系(P＜0.05)。结论：AP时腺泡细胞的凋亡是机体的一种自我保护机制，Bcl-2参与了腺泡细胞凋亡的调控。【关键词】 急性胰腺炎； 腺泡细胞凋亡； Bcl-2Apoptosis of Acinar Cells and Bcl-2 Expression in Rats with Acute Pancreatitis/XIE Rong-jun,ZHANG Shu-you,LI Feng,et al.//Medical Innovation of China,2012,9（11）:000-000【Abstract】 Objective:To explore the relationship between apoptosis of acinar cells and Bcl-2 expression in rats with acute pancreatitis. Methods:Thirty SD rats were randomly divided into three groups: group A(shame operation group), group B(acute edematous pancreatitis group) and group C(acute hemorrhagic necrotic group). Two kinds of pancreatitis model were induced by retrogradely injection of sodium deoxycholate. After 12 hours, the serum amylase activity, ascites, apoptosis of acinar cells and expression of Bcl-2 were measured, the general and histological changes of pancreas were evaluated by the corresponding score system. Results:⑴ The serum amylase activity, ascites volume, general and histological pathology score, positive rate of Bcl-2 in group C were significantly higher than B(P＜0.05), AI of acinar cells lower than B(P＜0.05). ⑵ In group B and C, AI were negatively correlated with general score and histological score(P＜0.05). Conclusion：The apoptosis of acinar cells is a self-protection mechanism of individuals with AP, Bcl-2 is involved with the regulation of the acinar cells apoptosis.【Key words】 Acute pancreatitis; Apoptosis of acinar cell; Bcl-2First-author’s address: Nanhua Affiliated Hospital of Nanhua University,Hengyang 421001,Chinadoi：10.3969/j.issn.1674-4985.2012.11.000急性胰腺